CD45RO Antibody (UCHL1) [Phycoerythrin]

Human PBMC were stained with (A) Mouse Anti-Human CD45RO PE-conjugated Monoclonal Antibody (Catalog # FAB10642P) or (B) Mouse IgG2A Isotype Control Antibody (IC003P) and Mouse Anti-Human CD45RA APC-conjugated Monoclonal Antibody. Staining was performed using our Staining Membrane-associated Proteins protocol.

Product Discontinued

CD45RO Antibody (UCHL1) [Phycoerythrin] Summary

• Immunogen: Cultured T cells from an IL-2-dependent T-cell line (CA1) prepared from human peripheral blood activated with influenza virus.
• Specificity: Detects human CD45RO in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD45RO Antibody (UCHL1) [Phycoerythrin]

• B220
• CD45 antigen
• CD45R
• CD45RO
• EC 3.1.3.48
• L-CA
• LY5
• protein tyrosine phosphatase, receptor type, C
• receptor-type tyrosine-protein phosphatase C
• T200 glycoprotein
• T200 leukocyte common antigen
• T200receptor type, c polypeptide

Background

CD45, previously called LCA (leukocyte common antigen), T200, or Ly5 in mice, is member C of the class 1 (receptor‑like) protein tyrosine phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180-220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1-3). Multiple splicing isoforms of exon 4 (A), 5 (B), and 6 (C) are expressed according to cell type, developmental stage and antigenic exposure (1-5). The longest form, CD45RABC (called B220 in mouse) is expressed on B lymphocytes, The shortest form, CD45R0, lacking exons 4, 5 and 6 which encode aa 34‑194, is expressed on memory cells, while intermediate sizes are expressed on other T cells (3, 4, 6). Human CD45 has a 40% and 41% sequence identity with mouse and rat respectively. The CD45R0 cDNA encodes 1145 amino acids (aa), including a 25 aa signal sequence, a 391 aa extracellular domain, 21 aa transmembrane sequence, and a 708 aa cytoplasmic domain that contains two phosphatase domains, D1 and D2. Only D1 has phosphatase activity. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6‑8). CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). Galectin interaction can be fine‑tuned by varying usage of the heavily O‑glycosylated spliced regions and sialylation of N‑linked carbohydrates (4, 9). Within the IS, CD45 dephosphorylates and negatively regulates the Src family kinase, Lck (8‑10). In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP‑1/CD148 (11‑14). CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7).

1. Anderson, J.N. et al. (2004) FASEB J. 18:8.
2. Streuli, M. et al. (1987) J. Exp. Med. 166:1548.
3. Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107.
4. Earl, L.A. and L.G. Baum (2008) Immunol. Cell Biol. 86:608.
5. Ralph, S.J. et al. (1987) EMBO J. 6:1251.
6. Falahti, R. and D. Leitenberg (2008) J. Immunol. 181:6082.
7. Tchilian, E.Z. and P.C.L. Beverley (2006) Trends Immunol. 27:146.
8. McNiell, L. et al. (2007) Immunity 27:425.
9. Chen, I-J. et al. (2007) J. Biol. Chem. 282:35361.
10. Freiberg, B.A. et al. (2002) Nat. Immunol. 3:911.
11. Zhu, J.W. et al. (2008) Immunity 28:183.
12. Huntington, N.D. et al. (2006) Nat. Immunol. 7:190.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for CD45RO Antibody (FAB10642P). (Showing 1 - 1 of 1 FAQ).

1. I cannot find any anti-mouse CD45RO for FACS. Have any of your anti-Human CD45RO been tested on mouse T cells?
   • Unfortunately, none of our CD45RO antibodies for Flow Cytometry have been validated on mouse. If you would be interested in testing the antibody on mouse, we would recommend Our Innovators Reward Program. Under the terms and conditions of this program we would be happy to provide you with a credit of equal or lesser value, in exchange for your online review including an image of either positive or negative staining for testing in a new species and/or application. Please contact us at innovators@novusbio.com with any questions regarding this program.