CD43/Sialophorin Antibody (R2/60) - BSA Free

Flow Cytometry: CD43/Sialophorin Antibody (R2/60) [NBP1-43413] - Analysis using the FITC conjugate of NBP1-43413. Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC and 0.06 ug of Rat IgM kappa Isotype Control FITC (left) or 0.06 ug of Anti-Mouse CD43 FITC (right).

Flow Cytometry: CD43/Sialophorin Antibody (R2/60) [NBP1-43413] - Staining of C57Bl/6 splenocytes with 0.25 ug of Rat IgM Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD43 Purified (filled histogram) followed by Anti-Rat IgM PE . Cells in the lymphocyte gate were used for analysis.

Flow Cytometry: CD43/Sialophorin Antibody (R2/60) [NBP1-43413] - Analysis using the Biotin conjugate of NBP1-43413. Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC and 0.25 ug of Rat IgM kappa Isotype Control Biotin (left) or 0.25 ug of Anti-Mouse CD43 Biotin (right) followed by Streptavidin PE.

• Reactivity: Mu
• Applications: WB, Flow, IP
• Clone: R2/60
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

CD43/Sialophorin Antibody (R2/60) - BSA Free Summary

• Immunogen: The immunogen for this antibody was CD43.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Rat
• Gene: SPN
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: This R2/60 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Reactivity Notes

This antibody is reactive to Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for CD43/Sialophorin Antibody (R2/60) - BSA Free

• CD43 antigen
• CD43
• CD43)
• Galactoglycoprotein
• GALGP
• GPL115
• Leukocyte sialoglycoprotein
• Leukosialin
• LSN
• Ly-48
• sialophorin (gpL115, leukosialin, CD43)
• Sialophorin
• SPN

Background

The R2/60 monoclonal antibody reacts with mouse CD43, also known as leukosialin and sialophorin. CD43 has glycoforms of approximately 115 and 130 kDa, and is expressed by an early B cell subset in BM, thymocytes and mature T cells, NK cells, myeloid lineage cells and platelets. CD43 is reported to bind to CD54 and play a role in adhesion and B cell survival. While the S7 and 1B11 monoclonal antibody clones detect only certain isoforms of CD43, data suggests that R2/60 detects the expression of both isoforms. It has been demonstrated that R2/60 stains the majority of CD4+ and CD8+ splenocytes, as well as a significant population of CD19+ splenocytes. However, the clones S7 and 1B11 stain only CD4+ and CD8+, or CD19+ splenocytes, respectively. This suggests that S7 and 1B11 recognize distinct epitopes which are differentially expressed by splenic B and T cells, whereas R2/60 recognizes an epitope shared by both isoforms of CD43.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPN