CD35 Antibody (E11) - Azide and BSA Free

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Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications ELISA
Clone
E11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

CD35 Antibody (E11) - Azide and BSA Free Summary

Immunogen
Intact human monocytes were used as immunogen to generate the CD35 antibody (Hogg et al, 1984).
Localization
Cell surface
Marker
Follicular Dendritic Cell Marker
Specificity
Recognizes a protein of 210-220kDa, which is identified as the complement receptor 1 (CR1)/CD35. This MAb is specific for a site in CR1 that is distal from the C3b-binding site, so that it is unable to block CR1 activity. This MAb is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This MAb labels follicular dendritic cells and follicular dendritic cell sarcoma.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CR1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1-5ug/ml for coating
Application Notes
Use in Western Blot has been reported in literature. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Theoretical MW
215 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Baboon, Cynomolgus Monkey, Rhesus Monkey.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS - BSA free
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD35 Antibody (E11) - Azide and BSA Free

  • C3BR
  • CD35
  • complement component (3b/4b) receptor 1 (Knops blood group)
  • CR1
  • KN

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CR1
Entrez