CD30 Ligand/TNFSF8 Antibody (RM153) - Chimeric - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: CD30 Ligand/TNFSF8 Antibody (RM153) - Chimeric [NBP3-11991] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of RG9-35 (NBP3-11991) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11991, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

• Reactivity: Mu
• Applications: Flow, ICC/IF, B/N, Func
• Clone: RM153
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

CD30 Ligand/TNFSF8 Antibody (RM153) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit CD30 Ligand/TNFSF8 Antibody (RM153) - Chimeric - Azide and BSA Free (NBP3-11991) is a recombinant monoclonal antibody validated for use in Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was raised by immunising Sprague Dawley rats with the CHO cells stably transfected with murine CD30 Ligand/TNFSF8 and fusing the splenocytes with P3U1 myeloma cells.
• Specificity: This antibody is specific for murine CD30 Ligand/TNFSF8, a 40-kDa type II transmembrane protein member of the TNF superfamily. Interaction of CD30 with CD30 Ligand/TNFSF8 plays an important role in T cell activation/costimulation.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TNFSF8
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Flow Cytometry
  • Functional Assay
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CD30 Ligand/TNFSF8 Antibody (RM153) - Chimeric - Azide and BSA Free

• CD153 antigen
• CD153
• CD30 antigen ligand
• CD30 Ligand
• CD30L
• CD30-L
• CD30LCD30 ligand
• CD30LGMGC138144
• TNFSF8
• tumor necrosis factor (ligand) superfamily, member 8
• tumor necrosis factor ligand superfamily member 8

Background

CD153 is a 39-40 kD type II membrane glycoprotein, also known as CD30 ligand or CD30L. This TNF superfamily member is expressed on activated T cells. CD153 has been reported to stimulate B-cell proliferation, antigen-specific antibody production, polyclonal immunoglobulin secretion, and T cell activation and proliferation. The RM153 antibody inhibits binding of CD153 to CD30.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNFSF8