![Western Blot: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Analysis of CD3G on mouse thymus tissue lysate using anti-CD3G antibody at 1/500 dilution.](http://images.novusbio.com/fullsize2/CD3 gamma Antibody (JB38-29)-Western Blot-NBP2-75435-img0004.jpg "Western Blot: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Analysis of CD3G on mouse thymus tissue lysate using anti-CD3G antibody at 1/500 dilution.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, IHC, IHC-P, IP |
| Clone | JB38-29 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Recombinant protein |
| Localization | Cell membrane. Membrane. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CD3G |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 20 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 1%BSA, 40%Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Diseases for CD3 gamma Antibody (NBP2-75435)Discover more about diseases related to CD3 gamma Antibody (NBP2-75435).
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PTMs for CD3 gamma Antibody (NBP2-75435)Learn more about PTMs related to CD3 gamma Antibody (NBP2-75435).
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| Gene Symbol | CD3G |
![Immunohistochemistry-Paraffin: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Analysis of paraffin-embedded human spleen tissue using anti-CD3G antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/CD3-gamma-Antibody-(JB38-29)-Immunohistochemistry-Paraffin-NBP2-75435-img0003.jpg "Immunohistochemistry-Paraffin: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Analysis of paraffin-embedded human spleen tissue using anti-CD3G antibody. Counter stained with hematoxylin.")
![Flow Cytometry: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Analysis of Jurkat cells with CD3G antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize2/CD3-gamma-Antibody-(JB38-29)-Flow-Cytometry-NBP2-75435-img0001.jpg "Flow Cytometry: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Analysis of Jurkat cells with CD3G antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.")
![Immunohistochemistry-Paraffin: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Analysis of paraffin-embedded human tonsil tissue using anti-CD3G antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/CD3-gamma-Antibody-(JB38-29)-Immunohistochemistry-Paraffin-NBP2-75435-img0002.jpg "Immunohistochemistry-Paraffin: CD3 gamma Antibody (JB38-29) [NBP2-75435] - Analysis of paraffin-embedded human tonsil tissue using anti-CD3G antibody. Counter stained with hematoxylin.")
![Flow Cytometry: CD3 delta Antibody (TS-1) [NBP2-22488] - Analysis of TCR Delta TCS1 in Jurkat cells (green) compared to an isotype control (blue).](http://images.novusbio.com/images2/CD3-delta-Antibody-(TS-1)-Flow-Cytometry-NBP2-22488-img0001.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0018.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/images2/HLA DQ Antibody (SPV-L3)-Flow Cytometry-NBP2-45041-img0001.jpg)
![Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.](http://images.novusbio.com/images2/CD4-Antibody-Western-Blot-NBP1-19371-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/images2/CD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-19371-img0005.jpg)
![Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.](http://images.novusbio.com/images2/CD4-Antibody-Immunohistochemistry-Paraffin-NBP1-19371-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of immersion fixed splenocytes. Primary antibody was used at a dilution of 10 ug/mL and incubated for 3 hours at room temperature.](http://images.novusbio.com/images2/CD8-Antibody-(53-6.7)-Immunocytochemistry-Immunofluorescence-NBP1-49045-img0011.jpg)
![Immunohistochemistry: CD8 Antibody (53-6.7) [NBP1-49045] - Histopathological analysis of spinal cord in EAE mice. Representative spinal cord sections from wild-type (WT), Asic1-/-, and Asic2-/- mice at 45 days after immunization. Sections of the spinal cord anterior horn were stained with hematoxylin and eosin (E&H) to assess inflammation and immunostained for MCHII, CD4+, CD8+ infiltrating cells. Image collected and cropped by CiteAb from the following publication (https://onlinelibrary.wiley.com/doi/abs/10.1111/ejn.14302), licensed under a CC-BY licence.](http://images.novusbio.com/images2/CD8-Antibody-(53-6.7)-Immunohistochemistry-NBP1-49045-img0016.jpg)
![Flow Cytometry: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by multiple staining.](http://images.novusbio.com/images2/CD8-Antibody-(53-6.7)-Flow-Cytometry-NBP1-49045-img0014.jpg)
![Western Blot: TCR alpha Antibody [NBP1-45883] - Analysis of TCRa expression in Jurkat cell lysate.](http://images.novusbio.com/images2/TCR alpha Antibody-Western Blot-NBP1-45883-img0002.jpg)
![Western Blot: HMBS Antibody [NBP1-87329] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/images2/HMBS-Antibody-Western-Blot-NBP1-87329-img0006.jpg)
![Immunohistochemistry-Paraffin: HMBS Antibody [NBP1-87329] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/images2/HMBS-Antibody-Immunohistochemistry-Paraffin-NBP1-87329-img0009.jpg)
![Western Blot: HMBS Antibody [NBP1-87329] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/HMBS-Antibody-Western-Blot-NBP1-87329-img0005.jpg)
![Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymus.](http://images.novusbio.com/images2/CD90-Thy1-Antibody-(OX-7)-Flow-Cytometry-NB100-65543-img0006.jpg)
![Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymus with MOUSE ANTI RAT CD90 (THY 1.1).](http://images.novusbio.com/images/CD90-Thy1 Antibody (OX-7)-Flow Cytometry-NB100-65543-img0001.jpg)
![Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymocytes.](http://images.novusbio.com/images2/CD90-Thy1-Antibody-(OX-7)-Flow-Cytometry-NB100-65543-img0002.jpg)
![Western Blot: Ets-1 Antibody (JM92-32) [NBP2-66826] - Analysis of ETS1 on different cell lysates using anti-ETS1 antibody at 1/1,000 dilution. Positive control: Line1: Jurkat Line2: K562 Line3: HepG2](http://images.novusbio.com/images2/Ets-1 Antibody (JM92-32)-Western Blot-NBP2-66826-img0005.jpg)
![Immunohistochemistry-Paraffin: Ets-1 Antibody (JM92-32) [NBP2-66826] - Analysis of paraffin-embedded mouse spleen tissue using anti-ETS1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/Ets-1-Antibody-(JM92-32)-Immunohistochemistry-Paraffin-NBP2-66826-img0004.jpg)
![Flow Cytometry: Ets-1 Antibody (JM92-32) [NBP2-66826] - Analysis of Jurkat cells with ETS1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/images2/Ets-1-Antibody-(JM92-32)-Flow-Cytometry-NBP2-66826-img0001.jpg)
![Western Blot: Calcium-binding-protein-P22 Antibody [NBP1-33736] - A. 50 ug mouse liver lysate/extract 12 % SDS-PAGE](http://images.novusbio.com/images2/Calcium-binding-protein-P22-Antibody-Western-Blot-NBP1-33736-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Calcium-binding-protein-P22 Antibody [NBP1-33736] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calcium binding protein P22 protein stained by Calcium binding protein P22 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/images2/Calcium-binding-protein-P22-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33736-img0009.jpg)
![Immunohistochemistry-Paraffin: Calcium-binding-protein-P22 Antibody [NBP1-33736] - Paraffin-embedded mouse lung. Calcium binding protein P22 antibody dilution: 1:500.](http://images.novusbio.com/images2/Calcium-binding-protein-P22-Antibody-Immunohistochemistry-Paraffin-NBP1-33736-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: ZAP70 Antibody (2F3.2) [NBP2-44502] - Immunofluorescence Analysis of PFA-fixed Jurkat cells labeled with ZAP70 Antibody (2F3.2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red).](http://images.novusbio.com/images2/ZAP70-Antibody-(2F3.2)-Immunocytochemistry-Immunofluorescence-NBP2-44502-img0006.jpg)
![Immunohistochemistry-Paraffin: ZAP70 Antibody (2F3.2) [NBP2-44502] - Human Tonsil stained with ZAP70 Monoclonal Antibody (2F3.2).](http://images.novusbio.com/images2/ZAP70-Antibody-(2F3.2)-Immunohistochemistry-Paraffin-NBP2-44502-img0001.jpg)
![Flow Cytometry: ZAP70 Antibody (2F3.2) [NBP2-44502] - Flow Cytometric Analysis of PFA-fixed Jurkat cells. ZAP70 Antibody (2F3.2) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/images2/ZAP70-Antibody-(2F3.2)-Flow-Cytometry-NBP2-44502-img0005.jpg)
![Western Blot: Adenine Nucleotide Translocator 2 Antibody [NBP2-57642] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/images2/Adenine-Nucleotide-Translocator-2-Antibody-Western-Blot-NBP2-57642-img0002.jpg)
![Immunohistochemistry-Paraffin: Adenine Nucleotide Translocator 2 Antibody [NBP2-57642] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/images2/Adenine-Nucleotide-Translocator-2-Antibody-Immunohistochemistry-Paraffin-NBP2-57642-img0004.jpg)
![Immunohistochemistry-Paraffin: Adenine Nucleotide Translocator 2 Antibody [NBP2-57642] - Staining of human cerebellum shows strong granular cytoplasmic positivity in Purkinje cells and cells in granular layer.](http://images.novusbio.com/images2/Adenine-Nucleotide-Translocator-2-Antibody-Immunohistochemistry-Paraffin-NBP2-57642-img0003.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")