CD3 epsilon Antibody (UCHT1R-FAB2-TEV/His) [Unconjugated]

Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CD3e FAB2 Recombinant Monoclonal Antibody (Catalog # MAB100R-FAB2) or (B) Mouse IgG1 isotype control antibody (MAB002), followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B) and Mouse anti-Human CD4 PE-conjugated Monoclonal Antibody (FAB3791P). Staining was performed using our staining membrane proteins protocol.

Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CD3e FAB2 Recombinant Monoclonal Antibody (Catalog # MAB100R-FAB2) or (B) Mouse IgG1 isotype control antibody (MAB002), followed by PE-conjugated Anti-His Antibody (IC050P) and Mouse anti-Human CD4 APC-conjugated Monoclonal Antibody (FAB3791A). Staining was performed using our staining membrane proteins protocol.

• Reactivity: Hu
• Applications: Flow
• Clone: UCHT1R-FAB2-TEV/His
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CD3 epsilon Antibody (UCHT1R-FAB2-TEV/His) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Human thymocytes
• Specificity: Detects human CD3 epsilon
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD3E
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD3 epsilon Antibody (UCHT1R-FAB2-TEV/His) [Unconjugated]

• CD3 epsilon
• CD3e antigen
• CD3e antigen, epsilon polypeptide (TiT3 complex)
• CD3e molecule, epsilon (CD3-TCR complex)
• CD3e
• CD3-epsilon
• FLJ18683
• T3E
• T-cell antigen receptor complex, epsilon subunit of T3
• T-cell surface antigen T3/Leu-4 epsilon chain
• T-cell surface glycoprotein CD3 epsilon chain
• TCRE

Background

CD3 epsilon is one of at least four invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

1. Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD3E