CD3 epsilon Antibody (SP7)

Product Discontinued

CD3 epsilon Antibody (SP7) Summary

• Immunogen: A synthetic peptide corresponding tointernal region of the epsilon chain of human CD3 epsilon protein.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CD3E
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:150
• Application Notes: This product is useful for Immunohistochemistry Paraffin. Antigen retrieval can be performed using a Citrate process.
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted by homology Bovine, chicken, canine, procine, rabbit .rat.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS (pH 7.5) and 1.0% BSA
• Preservative: 0.1% Sodium Azide
• Purity: Tissue culture supernatant

Notes

For a positive control use Colon carcinoma.

Alternate Names for CD3 epsilon Antibody (SP7)

• CD3 epsilon
• CD3e antigen
• CD3e antigen, epsilon polypeptide (TiT3 complex)
• CD3e molecule, epsilon (CD3-TCR complex)
• CD3e
• CD3-epsilon
• FLJ18683
• T3E
• T-cell antigen receptor complex, epsilon subunit of T3
• T-cell surface antigen T3/Leu-4 epsilon chain
• T-cell surface glycoprotein CD3 epsilon chain
• TCRE

Background

CD3 epsilon is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3 epsilon, one CD3 gamma, one CD3 delta, one CD3 zeta (CD247), and a T-cell receptor ( alpha/ beta or gamma/ delta) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. The UCHT1 antibody is able to induce T cell activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD3E