CD3 epsilon Antibody (PPT3) - Azide and BSA Free

Flow Cytometry: CD3 epsilon Antibody (PPT3) [NBP1-28225] - Analysis using the FITC conjugate of NBP1-28225. 1 ug/10^6 Peripheral blood mononuclear cells were isolated from heparinized pig blood on Ficoll-Hypaque density gradients and stained with mouse anti-pig CD3 epsilon-FITC.

Flow Cytometry: CD3 epsilon Antibody (PPT3) [NBP1-28225] - Analysis using the Biotin conjugate of NBP1-28225. Multiple staining of porcine peripheral blood lymphocytes.

• Reactivity: Po
• Applications: Flow
• Clone: PPT3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

CD3 epsilon Antibody (PPT3) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse CD3 epsilon Antibody (PPT3) - Azide and BSA Free (NBP1-28225) is a monoclonal antibody validated for use in Flow. Anti-CD3 epsilon Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified CD3 molecules from porcine thymus
• Specificity: Porcine CD3 epsilon, Mr 20 kDa
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD3E
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.1M BBS (pH 8.2)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD3 epsilon Antibody (PPT3) - Azide and BSA Free

• CD3 epsilon
• CD3e antigen
• CD3e antigen, epsilon polypeptide (TiT3 complex)
• CD3e molecule, epsilon (CD3-TCR complex)
• CD3e
• CD3-epsilon
• FLJ18683
• T3E
• T-cell antigen receptor complex, epsilon subunit of T3
• T-cell surface antigen T3/Leu-4 epsilon chain
• T-cell surface glycoprotein CD3 epsilon chain
• TCRE

Background

Porcine CD3 epsilon is a member of the T-cell receptor-associated CD3 complex. It is found on a subpopulation of thymocytes and on all pig T lymphocytes. MAb PPT3 is mitogenic when presented to peripheral blood mononuclear cells in immobilized form. (1)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD3 epsilon Antibody (NBP1-28225)(3)

Publications using NBP1-28225

• Sper RB, Proctor J, Lascina O et al. Allogeneic and xenogeneic lymphoid reconstitution in a RAG2 (-/-) IL2RG (y/-) severe combined immunodeficient pig: A preclinical model for intrauterine hematopoietic transplantation Frontiers in Veterinary Science 2022-10-14 [PMID: 36311661] (Flow Cytometry)
• Han J, Yoo I, Lee S et al. Interleukin-10 and Its Receptors at the Maternal-Conceptus Interface: Expression, Regulation, and Implication for Th2 Cytokine Predominance and Maternal Immune Tolerance in the Pig, a True Epitheliochorial Placentation Species Biology of reproduction 2022-03-25 [PMID: 35348632]
• AndriSicM, ZarkovicI, Sandor K Et al. Effects of immunostimulators of microbial origin on T cells of pigs vaccinated with attenuated vaccine against Aujeszky's disease Vet Immunol Immunopathol 2021-12-17 [PMID: 34920287] (FLOW, Porcine)
  
  Details:
  Citation using the Alexa Fluor 405 version of this antibody.

Bioinformatics

• Gene Symbol: CD3E