CD3 epsilon Antibody (PC3/188A) [mFluor Violet 450 SE]

CD3 epsilon Antibody (PC3/188A) [mFluor Violet 450 SE] [NBP2-54405MFV450] - Vial of mFluor Violet 450 conjugated antibody. mFluor Violet 450 is optimally excited at 406 nm by the Violet laser (405 nm) and has an emission maximum of 445 nm.

• Reactivity: Hu, Pm
• Applications: WB, Flow, ICC/IF, CyTOF-ready, IHC (-)
• Clone: PC3/188A
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: mFluor Violet 450 SE

CD3 epsilon Antibody (PC3/188A) [mFluor Violet 450 SE] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: A synthetic peptide corresponding to amino acids 156-168 of the cytoplasmic domain of human CD3- chain (Uniprot: P07766 )
• Localization: Cell surface and Cytoplasmic
• Marker: T-Cell Marker
• Specificity: Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD3E
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP2-54405. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Others not known.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CD3 epsilon Antibody (PC3/188A) [mFluor Violet 450 SE]

• CD3 epsilon
• CD3e antigen
• CD3e antigen, epsilon polypeptide (TiT3 complex)
• CD3e molecule, epsilon (CD3-TCR complex)
• CD3e
• CD3-epsilon
• FLJ18683
• T3E
• T-cell antigen receptor complex, epsilon subunit of T3
• T-cell surface antigen T3/Leu-4 epsilon chain
• T-cell surface glycoprotein CD3 epsilon chain
• TCRE

Background

CD3 epsilon is a subunit of CD3. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD3E