Mouse CD3 ELISA Kit (Colorimetric) Summary
This kit recognizes Mouse CD3 in samples. No significant cross-reactivity or interference between Mouse CD3 and analogues was observed.
|Standard Curve Range
0.39 - 25 ng/mL
CV% < 4.39%
CV% < 5.17%
ELISA Kit (Colorimetric)
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
- Biotinylated Detection Ab Diluent
- Certificate of Analysis
- Concentrated Biotinylated Detection Ab (100×)
- Concentrated HRP Conjugate (100×)
- Concentrated Wash Buffer (25×)
- HRP Conjugate Diluent
- Micro ELISA Plate(Dismountable)
- Plate Sealer
- Product Description
- Reference Standard & Sample Diluent
- Reference Standard
- Stop Solution
- Substrate Reagent
Alternate Names for Mouse CD3 ELISA Kit (Colorimetric)
- CD_antigen: CD3e
- CD3 antigen, delta subunit
- CD3d antigen
- CD3d antigen, delta polypeptide (TiT3 complex)
- CD3d molecule, delta (CD3-TCR complex)
- CD3e antigen
- CD3e antigen, epsilon polypeptide (TiT3 complex)
- CD3e molecule, epsilon (CD3-TCR complex)
- CD3g antigen
- CD3g antigen, gamma polypeptide (TiT3 complex)
- CD3g molecule, epsilon (CD3-TCR complex)
- CD3g molecule, gamma (CD3-TCR complex)
- T3DOKT3, delta chain
- T-cell antigen receptor complex, epsilon subunit of T3
- T-cell receptor T3 delta chain
- T-cell surface antigen T3/Leu-4 epsilon chain
- T-cell surface glycoprotein CD3 delta chain
- T-cell surface glycoprotein CD3 epsilon chain
CD3 (cluster of differentiation marker 3) is a multi-subunit transmembrane protein that is expressed on the surface of T-cells and forms a complex with the T-cell receptor (TCR) (1-3). CD3 consists of four distinct membrane protein isoforms: CD3-delta (delta), CD3-epsilon (epsilon), CD3-gamma, and CD3-zeta (1-3). The CD3 subunits organize into a complex containing three sets of dimers: CD3-epsilondelta, CD3-epsilongamma, CD3-zetazeta. The CD3 complex binds to the TCR heterodimer (alphabeta or gammadelta) to form the transmembrane TCR-CD3 complex (2-3). Structurally, the CD3 protein chains have an extracellular region, a transmembrane domain, and a cytoplasmic trail region (2-3). The CD3-epsilondelta and CD3-epsilongamma heterodimers also contain an extracellular immunoglobulin (Ig)-like domain, classifying them as part of the immunoglobulin superfamily (2-3). The cytoplasmic tail of each CD3 chain also contains one (delta, epsilon, gamma) or three (zeta) immunoreceptor tyrosine-based activation motifs (ITAMs), for a total of 10 ITAMs in the whole CD3 complex (2-3). Following, TCR binding to peptide major histocompatibility complex (p-MHC), the CD3 ITAMs are phosphorylated by the Src kinase Lck and are important for recruiting ZAP70 and initiating TCR signaling cascade activation (2). While similar in structure, the CD3 chains vary in length and molecular weight (4). The CD3-delta is 171 amino acids (aa) in length with a theoretical molecular weight of 18.9 kDa (4, 5). The CD3-epsilon is 204 aa long and has a theoretical molecular weight of 23 kDa (4, 5). CD3-gamma is 182 aa long with a theoretical molecular weight of 20.4 kDa (4, 5). Finally, CD3-zeta is 164 aa in length with a theoretical molecular weight of 18.6 kDa (4, 5).
CD3 proteins are expressed on the surface of thymocytes during thymocyte development, proliferation, and maturation to T-cells (4, 6, 7). During T-cell development CD4-CD8- double negative (DN) cells differentiate to CD4+CD8+ double positive (DP) cells before progressing to single positive (SP) CD4+ helper T-cells or CD8+ cytotoxic T-cells (4, 6, 7). As CD3 plays an important role in thymocyte development, it is understandable that CD3 defects and mutations in CD3 protein chains cause severe combined immunodeficiencies (SCIDs) (8). Additionally, a subset of CD3+ T-cells that co-express CD20 are described in a variety of diseases including rheumatoid arthritis, multiple sclerosis, CD20+ T-cell leukemia/lymphoma, and HIV (9). Clinical trials and animal models have shown that anti-CD3 monoclonal antibodies are a promising treatment modality for inflammatory disorders and autoimmune diseases, such as type I diabetes (10).
1. Chetty, R., & Gatter, K. (1994). CD3: structure, function, and role of immunostaining in clinical practice. The Journal of pathology. https://doi.org/10.1002/path.1711730404
2. Mariuzza, R. A., Agnihotri, P., & Orban, J. (2020). The structural basis of T-cell receptor (TCR) activation: An enduring enigma. The Journal of biological chemistry. https://doi.org/10.1074/jbc.REV119.009411
3. Kuhns, M. S., Davis, M. M., & Garcia, K. C. (2006). Deconstructing the form and function of the TCR/CD3 complex. Immunity. https://doi.org/10.1016/j.immuni.2006.01.006
4. Clevers, H., Alarcon, B., Wileman, T., & Terhorst, C. (1988). The T cell receptor/CD3 complex: a dynamic protein ensemble. Annual review of immunology. https://doi.org/10.1146/annurev.iy.06.040188.003213
5. Uniprot: CD3-delta (P04234), CD3-epsilon (P07766), CD3-gamma (P09693), CD3-zeta (P20963)
6. D'Acquisto, F., & Crompton, T. (2011). CD3+CD4-CD8- (double negative) T cells: saviours or villains of the immune response?. Biochemical pharmacology. https://doi.org/10.1016/j.bcp.2011.05.019
7. Dave V. P. (2009). Hierarchical role of CD3 chains in thymocyte development. Immunological reviews. https://doi.org/10.1111/j.1600-065X.2009.00835.x
8. Fischer, A., de Saint Basile, G., & Le Deist, F. (2005). CD3 deficiencies. Current opinion in allergy and clinical immunology. https://doi.org/10.1097/01.all.0000191886.12645.79
9. Chen, Q., Yuan, S., Sun, H., & Peng, L. (2019). CD3+CD20+ T cells and their roles in human diseases. Human immunology. https://doi.org/10.1016/j.humimm.2019.01.001
10. Kuhn, C., & Weiner, H. L. (2016). Therapeutic anti-CD3 monoclonal antibodies: from bench to bedside. Immunotherapy. https://doi.org/10.2217/imt-2016-0049
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed
for 6 months from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, WB
Applications: IHC, IHC-P, Simple Western, WB
Applications: ELISA, IHC, IHC-P, WB
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Applications: CompCytotox, CyTOF-ready, Flow, ICC, IHC, IP, Tstim
Applications: ICC, WB
Species: Hu, Mu, Rt
Applications: CyTOF-ready, Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, In vivo, WB
Species: Hu, Rt
Applications: Flow-CS, Flow, ICC/IF, IHC, IHC-P, Simple Western
Species: Hu, Mu(-), Rt(-)
Applications: IHC, IHC-Fr, IP
Species: Hu, Mu, Pm
Applications: IHC, IHC-P, WB
Publications for CD3 ELISA Kit (NBP2-75137) (0)
There are no publications for CD3 ELISA Kit (NBP2-75137).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for CD3 ELISA Kit (NBP2-75137) (0)
There are no reviews for CD3 ELISA Kit (NBP2-75137).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for CD3 ELISA Kit (NBP2-75137) (0)
Additional CD3 Products
Bioinformatics Tool for CD3 ELISA Kit (NBP2-75137)
Discover related pathways, diseases and genes to CD3 ELISA Kit (NBP2-75137). Need help?
Read the Bioinformatics Tool Guide
for instructions on using this tool.
Blogs on CD3.