CD3 Antibody

Images

 
Immunohistochemistry-Paraffin: CD3 Antibody [NBP1-72167] - Human tonsil tissue stained with Anti-CD3 antibody at 1:100 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate ...read more
Simple Western: CD3 Antibody [NBP1-72167] - Simple Western lane view shows a specific band for CD3 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more

Product Details

Summary
Product Discontinued
View other related CD3 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-72167
    • Availability
      Product Discontinued

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CD3 Antibody Summary

Immunogen
A synthetic peptide corresponding to extracellular domain of human CD3.
Localization
Cell Membrane
Specificity
This antibody recognizes CD3 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
CD3
Purity
Immunogen affinity purified
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Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
Sterile TBS (pH 7.2) with antibody stabilizer
Preservative
No Preservative
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 0.1-1 ug/ml
  • Simple Western 1:200
  • ELISA 0.01-0.1 ug/ml
  • Flow Cytometry 10-20 ug/10^6 cells
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 2-5 ug/ml
  • Immunoprecipitation 2-5 ug/ml
Application Notes
Use in ICC/IF was reported in the scientific literature (PMID: 23825096).In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-72167 in the following applications:

Alternate Names for CD3 Antibody

  • CD3 antigen, delta subunit
  • CD3
  • CD3d antigen
  • CD3d antigen, delta polypeptide (TiT3 complex)
  • CD3d molecule, delta (CD3-TCR complex)
  • CD3-DELTA
  • CD3e antigen
  • CD3e antigen, epsilon polypeptide (TiT3 complex)
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3-epsilon
  • CD3g antigen
  • CD3g antigen, gamma polypeptide (TiT3 complex)
  • CD3g molecule, epsilon (CD3-TCR complex)
  • CD3g molecule, gamma (CD3-TCR complex)
  • CD3G
  • CD3-GAMMA
  • FLJ17620
  • FLJ17664
  • FLJ18683
  • FLJ79544
  • FLJ94613
  • MGC138597
  • T3DOKT3, delta chain
  • T3E
  • T-cell antigen receptor complex, epsilon subunit of T3
  • T-cell receptor T3 delta chain
  • T-cell surface antigen T3/Leu-4 epsilon chain
  • T-cell surface glycoprotein CD3 delta chain
  • T-cell surface glycoprotein CD3 epsilon chain
  • TCRE

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD3 Antibody (NBP1-72167)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC
(2)
IHC-P
(1)
WB
(1)
All Applications
Filter By Species
Mouse
(3)
All Species

Reviews for CD3 Antibody (NBP1-72167) (0)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD3 Antibody (NBP1-72167). (Showing 1 - 2 of 2 FAQs).

  1. What the theoretical molecular weight for CD3 antibodies?
    • The TMW of CD3 antibodies is approximately 20 - 25 kDa.
  2. Can you guys recommend a CD3 antibody for use in Immunofluorescence in Rat please?
    • I would recommend NBP1-72167. It has been validated for use in Rat and it has also been validated for use in IHC so IF should not be a problem.

Secondary Antibodies

 

Isotype Controls

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CD3 NBP1-72167

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Blogs on CD3.

Transferrin and the blood brain barrier
Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

CD3 (OKT3) as a marker of immune response efficiency
Our immune system is a powerful defense mechanism against infection, however different variables can cause our immune response to work for or against us.  CD3 (cluster of differentiation 3) is one component of our immune signal response that is co...  Read full blog post.

The CD4 Antibody: More than Just a Cellular Marker
CD4 is a member of the cluster of differentiation family of proteins, mainly expressed on the surface of thymocytes and a specific subset of mature T-cells. CD4 antibody studies have also shown it expressed on monocytes, cortical cells, microglial cel...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol CD3
Entrez

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