Immunohistochemistry-Paraffin: CD3 Antibody [NBP1-72167] - Human tonsil tissue stained with Anti-CD3 antibody at 1:100 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate ...read more
Simple Western: CD3 Antibody [NBP1-72167] - Simple Western lane view shows a specific band for CD3 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Sterile TBS (pH 7.2) with antibody stabilizer
Immunogen affinity purified
Western Blot 0.1-1 ug/ml
Simple Western 1:200
ELISA 0.01-0.1 ug/ml
Flow Cytometry 10-20 ug/10^6 cells
Immunohistochemistry-Paraffin 2-5 ug/ml
Immunoprecipitation 2-5 ug/ml
Use in ICC/IF was reported in the scientific literature (PMID: 23825096).
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
23 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NBP1-72167 in the following applications:
Transferrin and the blood brain barrier Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i... Read full blog post.
MHC Class I and the Herpes Simplex Virus MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens. These molecules are highly polymorphic, meaning that they exist in multiple varian... Read full blog post.