CD28 [p Ser124, p Ser123] Antibody (JJ319)

Images

 
Flow Cytometry: CD28 [p Ser124, p Ser123] Antibody (JJ319) [NBP2-00000] - Analysis using the Biotin conjugate of NBP2-00000. Staining of rat splenocytes with Anti-Rat CD5 FITC and 0.25 micrograms of Mouse IgG1 k Isotype ...read more
Flow Cytometry: CD28 [p Ser124, p Ser123] Antibody (JJ319) [NBP2-00000] - Staining of Lou Rat splenocytes with 0.5 ug Mouse IgG1 Isotype Control Purified (NBP1-43319) (open histogram) or 0.5 ug Anti-Rat CD28 Purified ...read more
Flow Cytometry: CD28 [p Ser124, p Ser123] Antibody (JJ319) [NBP2-00000] - Analysis using the FITC conjugate of NBP2-00000. Staining of rat splenocytes with Anti-Rat CD5 APC and 0.5 ug of Mouse IgG1 K Isotype Control ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Flow
Clone
JJ319
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

CD28 [p Ser124, p Ser123] Antibody (JJ319) Summary

Immunogen
The immunogen for this antibody is CD28.
Modification
p Ser124, p Ser123
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD28
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
This JJ319 antibody has been tested by flow cytometric analysis of rat splenocyte suspensions. This can be used at less than or equal to 1.0 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The JJ319 antibody has also been reported for use in Immunoprecipitation. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Reactivity Notes

Rat

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CD28 [p Ser124, p Ser123] Antibody (JJ319)

  • CD28 antigen (Tp44)
  • CD28 antigen
  • CD28 molecule
  • CD28
  • MGC138290
  • T-cell-specific surface glycoprotein CD28
  • Tp44

Background

The JJ319 monoclonal antibody reacts with the rat CD28 molecule, expressed by thymocytes, mature T cells, and a subset of NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells. No reactivity of JJ319 is observed on mouse T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
MAB140
Species: Hu
Applications: Flow, IHC, CyTOF-ready, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
H00003117-M01
Species: Hu
Applications: WB, ELISA, PLA
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
NBP2-24867
Species: Hu, Mu
Applications: Flow, Func, In vitro, In vivo, CyTOF-ready, Flow-CS
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP2-25208
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
H00003669-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
DDX0131P-100
Species: Hu
Applications: Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-26666
Species: Mu
Applications: B/N, Flow, IP, In vitro
NBP2-12499
Species: Hu
Applications: IHC, IHC-P
NB100-91662
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-00000
Species: Rt
Applications: Flow

Publications for CD28 Antibody (NBP2-00000) (0)

There are no publications for CD28 Antibody (NBP2-00000).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CD28 Antibody (NBP2-00000) (0)

There are no reviews for CD28 Antibody (NBP2-00000). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD28 Antibody (NBP2-00000) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

FITC Labeled NBP2-00105

Additional CD28 Products

Bioinformatics Tool for CD28 Antibody (NBP2-00000)

Discover related pathways, diseases and genes to CD28 Antibody (NBP2-00000). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CD28 Antibody (NBP2-00000)

Discover more about diseases related to CD28 Antibody (NBP2-00000).
 

Pathways for CD28 Antibody (NBP2-00000)

View related products by pathway.

PTMs for CD28 Antibody (NBP2-00000)

Learn more about PTMs related to CD28 Antibody (NBP2-00000).

Blogs on CD28.

Thomson Reuters Predicts 2016 Nobel Prize Winners
Here at Bio-Techne we always look forward to the annual announcements of winners of the highly coveted Nobel Prize – the greatest award in science. How can you go about predicting which scientists might be in line for a life-changing phone-call fro...  Read full blog post.

CD80: A co-stimulator of T cell activation
CD80 is a 60kD single chain type I transmembrane glycoprotein that is a member of the immunoglobulin family. CD80 is expressed on activated B- and T-lymphocytes, as well as a subpopulation of previously activated B-cells, but not on the majority of re...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CD28 [p Ser124, p Ser123] Antibody (JJ319) and receive a gift card or discount.

Bioinformatics

Gene Symbol CD28
Entrez