CD28 Antibody (29704-1) [Unconjugated]

Human CD28 recombinant antibody enhances IL-2 secretion in Jurkat cells (immortalized human T cell line) treated with 5 ng/mL phorbol myristate acetate (PMA) and 0.9 µM calcium ionomycin. Antibody-mediated stimulation occurred in a dose-dependent manner, as measured using the Quantikine Human IL-2 ELISA kit. The ED50 for this effect is 5.00-50.0 ng/mL.

• Reactivity: Hu
• Applications: Func
• Clone: 29704-1
• Host: Human
• Conjugate: Unconjugated

CD28 Antibody (29704-1) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody. See GMP Antibody Protocols!
• Immunogen: Human T lymphocytes
• Specificity: Detects human CD28 in direct ELISAs
• Details of Functionality: Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. Freeman, G.J. et al. (1993) Science 262:909. The ED₅₀ for this effect is 5.00-50.0 ng/mL
• Source: N/A
• Isotype: IgG1
• Host: Human
• Purity: ≥95%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining
• Purity Statement: Protein G purified from cell culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • T Cell Stimulation 3 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • A minimum of 12 months when stored at ≤ -20 °C, as supplied. Refer to lot specific COA for the Use by Date.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
• Purity: ≥95%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

Full terms and conditions of sale can be found online in the Protein Sciences Segment T&Cs at: Terms & Conditions.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD28 Antibody (29704-1) [Unconjugated]

• CD28 antigen (Tp44)
• CD28 antigen
• CD28 molecule
• CD28
• MGC138290
• T-cell-specific surface glycoprotein CD28
• Tp44

Background

The Bio-Techne CD28 GMP antibody is derived from the 15e8 clone. CD28 is a critical protein expressed on the surface of T cells that provides a co-stimulatory signal necessary for T cell activation and proliferation. CD28 is structurally similar to CTLA-4, with both molecules exhibiting structural homology to the immunoglobulin (Ig) gene superfamily. CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant co-stimulatory pathways that regulate T and B cell responses. Both CD28 and CTLA-4 are composed of a single Ig V-like extracellular domain, a transmembrane domain, and an intracellular domain. CD28 and CTLA-4 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.