CD277/BTN3A1 Antibody (BT3.1) - BSA Free

Flow Cytometry: CD277/BTN3A1 Antibody (BT3.1) [NBP2-00227] - Staining of normal human peripheral blood cells with 0.5 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.5 ug of Anti-Human CD277 Purified (filled histogram). Cells in the lymphocyte gate were used for analysis.

• Reactivity: Hu
• Applications: Flow, IP
• Clone: BT3.1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

CD277/BTN3A1 Antibody (BT3.1) - BSA Free Summary

• Immunogen: The immunogen for this antibody is CD277 (B7 family, Butyrophilin).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BTN3A1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunoprecipitation 1:10-1:500
• Application Notes: This BT3.1 (20.1, BT3.1) antibody has been tested by flow cytometric analysis of normal human peripheral blood mononuclear cells. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This T3.1 (20.1, BT3.1) antibody has been reported for use in immunoprecipitation. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD277/BTN3A1 Antibody (BT3.1) - BSA Free

• BT3.1
• BTF5
• BTN3A1
• butyrophilin, subfamily 3, member A1
• CD277
• dJ45P21.3 (butyrophilin, subfamily 3, member A1)

Background

The BT3.1 monoclonal antibody recognizes BT3.1, BT3.2 and BT3.3. The BT family of proteins is a subgroup of the Ig superfamily (IgSF). BT is a glycoprotein that forms a major component of the milk fat globule molecule. In addition to BT, there are six other genes that have been discovered which have been sub-divided into the BT2 (BT2.1, BT2.2, and BT2.3) and BT3 (BT3.1, BT3.2, and BT3.3) families. BT3.1, BT3.2, and BT3.3 share 95% mRNA identity. BT3 molecules are constitutively expressed on the cell surface of antigen-presenting cells, and the IgV-like domain of BT3.1 is sufficient for interaction with its counter-receptor. BT3.1 is expressed by T cells, B cells and CD14+ cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BTN3A1