CD27/TNFRSF7 Antibody (LG.7F9) [FITC]

Images

 
Flow Cytometry: CD27/TNFRSF7 Antibody (LG.7F9) [FITC] [NBP1-44021] - Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC and 0.25 micrograms of Armenian Hamster IgG Isotype Control FITC (left) or 0.25 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications Flow
Clone
LG.7F9
Clonality
Monoclonal
Host
Armenian Hamster
Conjugate
FITC

CD27/TNFRSF7 Antibody (LG.7F9) [FITC] Summary

Immunogen
The immunogen for this antibody was CD27.
Isotype
IgG
Clonality
Monoclonal
Host
Armenian Hamster
Gene
CD27
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
The LG.7F9 antibody has been tested by flow cytometric analysis of mouse splenocyte cell suspensions. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Reactivity Notes

This antibody is reactive to Mouse, Rat, Human.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
Preservative
0.09% Sodium Azide
Purity
Protein A or G purified

Alternate Names for CD27/TNFRSF7 Antibody (LG.7F9) [FITC]

  • CD27 antigen
  • CD27 molecule
  • CD27
  • MGC20393
  • S152CD27L receptor
  • T cell activation antigen CD27
  • T cell activation antigen S152
  • T14
  • TNFRSF7
  • TNFRSF7T-cell activation antigen CD27
  • Tp55
  • Tumor necrosis factor receptor superfamily member 7
  • tumor necrosis factor receptor superfamily, member 7

Background

The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46-CD8- precursors. CD27 binds to CD70 and, through this interaction, plays an important role in T cell-B cell interaction. It has been reported that triggering CD27 plays an important role in the maturation of CD4+ and CD8+ effector cells. LG.7F9 cross-reacts with human and rat CD27.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD27
Entrez