CD24 Antibody (CLB-134)

Product Discontinued

CD24 Antibody (CLB-134) Summary

• Immunogen: The antibody reacts with the CD24 antigen.
• Specificity: The monoclonal antibody is directed agianst the (45, 55, 65 kD) CD24-antigen, which is expressed on virtually all B-cells: early B-cell precursors, pre-pre-B-cells, pre-B-cells, B-cells, intermediate B-cells, mature B-cells and some plasmacytoid cells. All these cells are positive while plasmacells are negative. The monoclonal antibody reacts with human granulocytes and thier precursors (from promyelocytic stage). The monoclonal antibody also reacts with virtually all pre-B-cell lines and Burkitt cell lines, but the antigen is only expressed on 50% of the B-cell lymphoblatoid cell lines. Non T-ALL, B-cell NHL and 50% of myelomas as well as a subpopulation of AML are found to be positive. Hairy Cell Leukaemia is found to be weakly positive.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD24
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
• Application Notes: Identification of B-CLL. Immunophenotyping of Hairy Cell Leukaemia. Cell studies on B-cells.Indirect immunofluorescence staining with analysis by cytofluorometry or fluorescence microscopy

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline, pH 7.4, with protein base
• Preservative: Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for CD24 Antibody (CLB-134)

• CD 24
• CD24 antigen (small cell lung carcinoma cluster 4 antigen)
• CD24 antigen
• CD24 molecule
• CD24
• CD24A
• CD24Asignal transducer CD24
• FLJ22950
• FLJ43543
• MGC75043
• Small cell lung carcinoma cluster 4 antigen

Background

This gene encodes a sialoglycoprotein that is expressed on mature granulocytes and in many B cells. The encoded protein is anchored via a glycosyl phosphatidylinositol (GPI) link to the cell surface. An alignment of this gene's sequence finds genomic locations with similarity on chromosomes 3p26, 15q21, 15q22, 20q11.2 and Yq11.1. Whether transcription, and corresponding translation, occurs at each of these other genomic locations needs to be experimentally determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD24