CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.25) - BSA Free

Flow Cytometry: CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.25) [NBP1-50070] - Surface staining of human peripheral blood cells with anti-human CD229 (HLy9.25) PE.

Flow Cytometry: CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.25) [NBP1-50070] - Surface staining of human peripheral blood cells with anti-human CD229 (HLy9.25) APC.

Surface staining pattern of human peripheral blood stained using anti-human CD229 (HLy9.25) purified antibody (concentration in sample 16 ug/ml) GAM APC.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IP, CyTOF-ready
• Clone: HLy9.25
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.25) - BSA Free Summary

• Description: Novus Biologicals Mouse CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.25) - BSA Free (NBP1-50070) is a monoclonal antibody validated for use in WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: CD299-transfected 300.19 pre-B cell line
• Specificity: The mouse monoclonal antibody HLy9.25 (also known as HLy9.1.25) recognizes Lymphocyte Antigen 9, a 100-120 kDa cell surface glycoprotein expressed on T and B cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LY9
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 6 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:50
  • Western Blot 1:1000
• Application Notes: Functional Application: Regulation of activation. This antibody is CyTOF ready.
• Theoretical MW: 120 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.25) - BSA Free

• CD229 antigen
• CD229
• cell-surface molecule Ly-9
• hly9
• Ly9
• lymphocyte antigen 9Cell surface molecule Ly-9
• mLY9
• SLAMF3
• T-lymphocyte surface antigen Ly-9

Background

CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LY9