CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.25)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, Func, ICC/IF, IP, CyTOF-ready
Clone
HLy9.25
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.25) Summary

Immunogen
CD299-transfected 300.19 pre-B cell line
Specificity
The mouse monoclonal antibody HLy9.25 (also known as HLy9.1.25) recognizes Lymphocyte Antigen 9, a 100-120 kDa cell surface glycoprotein expressed on T and B cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LY9
Purity
Protein A purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 6 ug/ml
  • Functional
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:50
  • CyTOF-ready
Application Notes
Functional Application: Regulation of activation

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.25)

  • CD229 antigen
  • CD229
  • cell-surface molecule Ly-9
  • hly9
  • Ly9
  • lymphocyte antigen 9Cell surface molecule Ly-9
  • mLY9
  • SLAMF3
  • T-lymphocyte surface antigen Ly-9

Background

CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol LY9
Entrez