CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.1.25)

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, Func, IP, CyTOF-ready
Clone
HLy9.1.25
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.1.25) Summary

Immunogen
Mouse pre-B cell line 300.19 transfected with CD229 cDNA
Specificity
Recognizes the human CD229 cell surface antigen, a 100-120kD cell surface glycoprotein that is the homologue of murine Ly-9. CD229 is a member of the immunoglobulin superfamily and may have a function in cell signalling. The antigen is expressed at low levels by mature T and B lymphocytes and at higher levels by single-positive thymocytes. Expression at variable levels is also seen in some cell lines, such as Daudi, U937 and HUT78. 19 transfected with CD229 cDNA
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LY9
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry 1:10-1:1000
  • Functional
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
Application Notes
The antibody can be used for flow cytometry and immunoprecipitation.Flow cytometry: Use 10ul of the suggested working dilution (1/50-1/100) to label 106 cells in 100ul or 100ul whole blood. Use in western blot and functional reported in scientific literature (PMID 27145901).
Publications
Read Publication using
NBP1-42422 in the following applications:

  • 1 publication
  • 1 publication
  • IP
    1 publication
  • WB
    1 publication

Reactivity Notes

Mouse. Human reactivity reported in scientific literature (PMID: 27145901). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.1.25)

  • CD229 antigen
  • CD229
  • cell-surface molecule Ly-9
  • hly9
  • Ly9
  • lymphocyte antigen 9Cell surface molecule Ly-9
  • mLY9
  • SLAMF3
  • T-lymphocyte surface antigen Ly-9

Background

LY9 belongs to the SLAM family of immunomodulatory receptors (see SLAMF1; MIM 603492) and interacts with the adaptor molecule SAP (SH2D1A; MIM 300490) (Graham et al., 2006 [PubMed 16365421]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (NBP1-42422)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: FLOW, Func, IP, WB.


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FLOW
(1)
Func
(1)
IP
(1)
WB
(1)
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Bioinformatics

Gene Symbol LY9
Entrez