CD21 Antibody (Bu33) [Alexa Fluor® 647]

Flow Cytometry: CD21 Antibody (Bu33) [Alexa Fluor 647] [NB100-63574AF647] - A surface stain was performed on human peripheral blood lymphocytes with CD21 (Bu33) antibody NB100-63574AF647 and a matched isotype control NBP2-27287AF647. Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-26646.

Flow Cytometry: CD21 Antibody (Bu33) [Alexa Fluor 647] [NB100-63574AF647] - Immunophenotyping with Canine whole blood. image courtesy of customer.

• Reactivity: Hu
• Applications: Flow
• Clone: Bu33
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

CD21 Antibody (Bu33) [Alexa Fluor® 647] Summary

• Additional Information: Clone Bu33 was used by HLDA to establish CD designation.
• Immunogen: Made to Human CD21
• Marker: Histiocytic Marker
• Specificity: NB100-63574 recognizes human CD21, a 145kD cell surface glycoprotein expressed by mature B lymphocytes, follicular dendritic cells, subsets of normal thymocytes and subsets of T cells. The CD21 molecule acts as a receptor for CD23, for complement components C3d, C3dg and iC3b and for Epstein Barr Virus. CD21 is part of the signalling complex that is linked to CD19, CD81 and phosphatidylinositol-3-kinase.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CR2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Reactivity Notes

Predicted cross-reactivities: Equine

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CD21 Antibody (Bu33) [Alexa Fluor® 647]

• C3DR
• C3DRSLEB9
• CD21 antigen
• CD21
• Complement C3d receptor
• complement component (3d/Epstein Barr virus) receptor 2
• complement receptor type 2
• CR2
• EBV receptor
• Epstein-Barr virus receptor

Background

CD21 (complement receptor 2, CR2) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to folicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for CD21 Antibody (NB100-63574AF647) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Canine.

reviewed by: A G Flow Canine 02/27/2017

Summary

• Application: Flow Cytometry
• Sample Tested: Dog whole blood
• Species: Canine
• Lot: 0307-022217-af647

Bioinformatics

• Gene Symbol: CR2