CD164 Antibody (502021) [Unconjugated]

CXCL12-treated PC-3 human prostate cancer cell line was stained with Mouse Anti-Human CD164 Monoclonal Antibody (Catalog # MAB5790, filled histogram) or isotype control antibody (Catalog # MAB007, open histogram), followed by APC-conjugated anti-mouse antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 502021
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CD164 Antibody (502021) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human CD14
  Asp24-Leu197
  Accession # Q04900
• Specificity: Detects human CD164 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CD164 is observed.
• Source: N/A
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD164
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD164 Antibody (502021) [Unconjugated]

• CD164 antigen
• CD164 molecule, sialomucin
• CD164
• Endolyn
• MGC-24
• MGC-24v
• MUC-24
• Multi-glycosylated core protein 24
• sialomucin

Background

CD164, also known as endolyn, MGC-24, and MUC-24, is an 80‑100 kDa transmembrane sialomucin protein that is expressed by epithelial cells, T and B cells, monocytes, hematopoietic progenitor cells, and activated basophils. CD164 functions as an adhesion molecule and a negative regulator of cell proliferation. A soluble isoform is generated by alternate splicing. CD164 was identified as a potential regulator of homing in a prostate cancer screening for CXCL12-responsive adhesion molecules (1). Within the ECD, human CD164 shares approximately 53% sequence identity with mouse and rat CD164.

1. Havens, A.M. et al. (2006) BMC Cancer 2006, 6:195.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD164
• Uniprot:
  • Q04900()