CD161/NK1.1 Antibody (PK136) [Unconjugated]

Mouse C57BL/6 splenocytes were stained with Mouse Anti-Mouse CD161/NK1.1 Monoclonal Antibody (Catalog # MAB76141, filled histogram) or Mouse IgG2A Isotype Control (Catalog # MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Histograms were gated on Rat Anti-Mouse NKp46/NCR1 PE-conjugated Monoclonal Antibody (Catalog # FAB22252P) positive cells. View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: PK136
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CD161/NK1.1 Antibody (PK136) [Unconjugated] Summary

• Immunogen: NK-1+ cells from mouse spleen and bone marrow
  Accession # AAH61168
• Specificity: Detects mouse CD161/NK1.1 in Flow Cytometry.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD161/NK1.1 Antibody (PK136) [Unconjugated]

• CD161 antigen
• CD161
• CD161/NK1.1
• CD161c
• CLEC5BC-type lectin domain family 5 member B
• HNKR-P1a
• killer cell lectin-like receptor subfamily B member 1
• killer cell lectin-like receptor subfamily B, member 1
• Klrb1c
• Ly55c
• Ly-59
• Natural killer cell surface protein P1A
• NKR-P1
• NKR-P1AMGC138614
• NKRP1ANKR

Background

NK-1.1 surface antigen is encoded by the NKR-P1B/NKR-P1C gene, also known as CD161b/CD161c and Ly-55. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN- gamma production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers. The PK136 antibody has been reported to deplete NK cells, induce redirected lysis, block NK cell function, and induce NK cell proliferation.

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • AAH61168()