CD14 Antibody (UCH-M1) - BSA Free

Images

 
Immunohistochemistry-Frozen: CD14 Antibody (UCH-M1) [NB100-2723] - Image from the Alexa Fluor 647 version of this antibody. Frozen human spleen stained for CD14 (red) and counter-stained with DAPI (bleu).

Product Details

Summary
Product Discontinued
View other related CD14 Primary Antibodies

Order Details


    • Catalog Number
      NB100-2723
    • Availability
      Product Discontinued

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CD14 Antibody (UCH-M1) - BSA Free Summary

Additional Information
Clone UCH-M1 was used by HLDA to establish CD designation.
Immunogen
Thymocytes and peripheral blood leucocytes (PBL)
Localization
Cytoplasmic. Attached to the membrane by a GPI anchor.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CD14
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation
  • CyTOF-ready
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen
Application Notes
UCH-M1 activates complement. This antibody is CyTOF ready.
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
CD14 Overexpression Lysate
Publications
Read Publication using NB100-2723.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for CD14 Antibody (UCH-M1) - BSA Free

  • CD14 antigen
  • CD14 molecule
  • CD14
  • monocyte differentiation antigen CD14
  • Myeloid cell-specific leucine-rich glycoprotein

Background

CD14 is a single copy gene encoding 2 protein forms: a 50 to 55 kDa glycosylphosphatidylinositol anchored membrane protein (mCD14) and a monocyte or liver derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram negative shock. CD14 also appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD14 Antibody (NB100-2723)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for CD14 Antibody (NB100-2723) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Diseases for CD14 Antibody (NB100-2723)

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Research Areas for CD14 Antibody (NB100-2723)

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Blogs on CD14.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

CD14 - TLR4 is my friend in battle against infections!
CD14 is a well-characterized cell-activating receptor for lipopolysaccharide-binding protein (LBP) and peptidoglycan. It is an important modulator for lipopolysaccharide (LPS)-dependent signaling and is a component of the multi-protein complex contain...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

mFluor Violet Conjugated Antibodies

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Bioinformatics

Gene Symbol CD14
Entrez