CD109 Antibody (496920) [Unconjugated]

A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human CD109 Monoclonal Antibody (Catalog # MAB4385, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

CD109 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CD109 Monoclonal Antibody (Catalog # MAB4385) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: Flow, IHC, CyTOF-ready
• Clone: 496920
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CD109 Antibody (496920) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CD109
  Val22-Ser1268 (Tyr703Ser & Thr1241Met)
  Accession # Q6YHK3
• Specificity: Detects human CD109 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD109
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD109 Antibody (496920) [Unconjugated]

• 150 kDa TGF-beta-1-binding protein
• activated T-cell marker CD109
• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7
• CD109 antigen (Gov platelet alloantigens)
• CD109 antigen
• CD109 molecule
• CD109
• CPAMD7
• CPAMD7r150
• DKFZp762L1111
• FLJ38569
• FLJ41966
• Gov platelet alloantigens
• p180
• Platelet-specific Gov antigen
• RP11-525G3.1

Background

CD109 is a GPI-anchored member of the alpha-2-macroglobulin (A2M) and complement family of proteins (1). Mature human CD109 contains a bait region with recognition sequences for multiple proteases, an internal thioester bond, and a domain similar to the receptor binding domain of A2M (2). Cleavage of A2M family proteins within the bait region activates the thioester bond to promote covalent bonding to nucleophilic groups in adjacent molecules (3, 4). Within the region included in this recombinant protein, human CD109 shares 71-73% amino acid (aa) sequence identity with mouse and rat CD109. It shares 27-33% aa sequence identity with A2M and complement factors C3, C4, and C5. Alternate splicing of human CD109 generates two isoforms with short deletions and one that is truncated within the bait region. CD109 is expressed on activated T cells and platelets, hematopoietic stem cells, megakaryocyte precursors, vascular endothelial cells, basal and myoepithelial cells of secretory glands, and squamous cell carcinomas (2, 5-9). It is produced as a 170-180 kDa glycoprotein that is autocatalytically processed to 150 kDa and 120 kDa forms (2, 6, 10). CD109 on keratinocytes binds TGF-beta and associates with TGF-beta RI and TGF-beta RII, resulting in inhibition of TGF-beta signaling (11). Polymorphisms of CD109 include the platelet-specific Gov antigen and the blood group ABH antigens (12, 13). Alloantibodies directed against these antigens result in unsuccessful platelet transfusions, neonatal alloimmune thrombocytopenia, and posttransfusion purpura (14).

1. Travis, J. and G.S. Salvesen (1983) Annu. Rev. Biochem. 52:655.
2. Lin, M. et al. (2002) Blood 99:1683.
3. Christensen, U. and L. Sottrup-Jensen (1984) Biochemistry 23:6619.
4. Wallis, R. et al. (2007) J. Biol. Chem. 282:7844.
5. Murray, L.J. et al. (1999) Exp. Hematol. 27:1282.
6. Haregewoin, A. et al. (1994) Cell. Immunol. 156:357.
7. Hasegawa, M. et al. (2007) Pathol. Int. 57:245.
8. Brashem-Stein, C. et al. (1988) J. Immunol. 140:2330.
9. Hashimoto, M. et al. (2004) Oncogene 23:3716.
10. Solomon, K.R. et al. (2004) Gene 327:171.
11. Finnson, K.W. et al. (2006) FASEB J. 20:1525.
12. Schuh, A.C. et al. (2002) Blood 99:1692.
13. Kelton, J.G. et al. (1998) J. Lab. Clin. Med. 132:142.
14. Rozman, P. (2002) Transpl. Immunol. 10:165.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD109 Antibody (MAB4385)(4)

Publications using MAB4385

• Zhou, S;Sosina, OA;Bovijn, J;Laurent, L;Sharma, V;Akbari, P;Forgetta, V;Jiang, L;Kosmicki, JA;Banerjee, N;Morris, JA;Oerton, E;Jones, M;LeBlanc, MG;Regeneron Genetics Center, ;Idone, V;Overton, JD;Reid, JG;Cantor, M;Abecasis, GR;Goltzman, D;Greenwood, CMT;Langenberg, C;Baras, A;Economides, AN;Ferreira, MAR;Hatsell, S;Ohlsson, C;Richards, JB;Lotta, LA; Converging evidence from exome sequencing and common variants implicates target genes for osteoporosis Nature genetics 2023-08-01 [PMID: 37558884] (ICC, Human)
• P Filppu, JT Ramanathan, KJ Granberg, E Gucciardo, H Haapasalo, K Lehti, M Nykter, V Le Joncour, P Laakkonen CD109-GP130 interaction drives glioblastoma stem cell plasticity and chemoresistance through STAT3 activity JCI Insight, 2021-05-10;6(9):. 2021-05-10 [PMID: 33986188] (IHC, PLA, Human)
• Y Tian, G Seumois, LM De-Oliveir, J Mateus, S Herrera-de, C Kim, D Hinz, NDS Goonawardh, AD de Silva, S Premawansa, G Premawansa, A Wijewickra, A Balmaseda, A Grifoni, P Vijayanand, E Harris, B Peters, A Sette, D Weiskopf Molecular Signatures of Dengue Virus-Specific IL-10/IFN-gamma Co-producing CD4�T Cells and Their Association with Dengue Disease Cell Rep, 2019-12-24;29(13):4482-4495.e4. 12/24/2019 [PMID: 31875555]
• Emori , Makoto, Tsukahara , Tomohide, Murase , Masaki, Kano , Masanobu, Murata , Kenji, Takahashi , Akari, Kubo , Terufumi, Asanuma , Hiroko, Yasuda , Kazuyo, Kochin , Vitaly, Kaya , Mitsunor, Nagoya , Satoshi, Nishio , Jun, Iwasaki , Hiroshi, Sonoda , Tomoko, Hasegawa , Tadashi, Torigoe , Toshihik, Wada , Takuro, Yamashita , Toshihik, Sato , Noriyuki High expression of CD109 antigen regulates the phenotype of cancer stem-like cells/cancer-initiating cells in the novel epithelioid sarcoma cell line ESX and is related to poor prognosis of soft tissue sarcoma. PLoS ONE, 2013-12-20;8(12):e84187. 2013-12-20 [PMID: 24376795] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CD109
• Uniprot:
  • Q6YHK3()