CD109 Antibody (496920) [Phycoerythrin]

A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse CD109 PE-conjugated Monoclonal Antibody (Catalog # FAB4385P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Mouse splenocytes treated with PMA and Calcium Ionomycin were stained with Mouse Anti-Human/Mouse CD109 PE-conjugated Monoclonal Antibody (Catalog # FAB4385P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Human peripheral blood mononuclear cells (PBMCs) treated with 2 ug/mL PHA for 5 days were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and (A) Mouse Anti-Human CD109 PE-conjugated Monoclonal Antibody (Catalog # FAB4385P) or (B) PE-conjugated Mouse IgG2A isotype control (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu, Mu
• Applications: Flow
• Clone: 496920
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

CD109 Antibody (496920) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CD109
  Val22-Ser1268 (Tyr703Ser & Thr1241Met)
  Accession # Q6YHK3
• Specificity: Detects human CD109 in direct ELISAs. Detects human and mouse CD109 in flow cytometry
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD109
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD109 Antibody (496920) [Phycoerythrin]

• 150 kDa TGF-beta-1-binding protein
• activated T-cell marker CD109
• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7
• CD109 antigen (Gov platelet alloantigens)
• CD109 antigen
• CD109 molecule
• CD109
• CPAMD7
• CPAMD7r150
• DKFZp762L1111
• FLJ38569
• FLJ41966
• Gov platelet alloantigens
• p180
• Platelet-specific Gov antigen
• RP11-525G3.1

Background

CD109 is a GPI-anchored member of the alpha -2-Macroglobulin (A2M) and complement family of proteins (1). Mature human CD109 contains a bait region with recognition sequences for multiple proteases, an internal thioester bond, and a domain similar to the receptor binding domain of A2M (2). Cleavage of A2M family proteins within the bait region activates the thioester bond to promote covalent bonding to nucleophilic groups in adjacent molecules (3, 4). Within the region included in this recombinant protein, human CD109 shares 71-73% amino acid (aa) sequence identity with mouse and rat CD109. It shares 27-33% aa sequence identity with A2M and complement factors C3, C4, and C5. Alternate splicing of human CD109 generates two isoforms with short deletions and one that is truncated within the bait region. CD109 is expressed on activated T cells and platelets, hematopoietic stem cells, megakaryocyte precursors, vascular endothelial cells, basal and myoepithelial cells of secretory glands, and squamous cell carcinomas (2, 5-9). It is produced as a 170-180 kDa glycoprotein that is autocatalytically processed to 150 kDa and 120 kDa forms (2, 6, 10). CD109 on keratinocytes binds TGF-beta and associates with TGF-beta RI and TGF-beta RII, resulting in inhibition of TGF-beta signaling (11). Polymorphisms of CD109 include the platelet-specific Gov antigen and the blood group ABH antigens (12, 13). Alloantibodies directed against these antigens result in unsuccessful platelet transfusions, neonatal alloimmune thrombocytopenia, and posttransfusion purpura (14).

1. Travis, J. and G.S. Salvesen (1983) Annu. Rev. Biochem. 52:655.
2. Lin, M. et al. (2002) Blood 99:1683.
3. Christensen, U. and L. Sottrup-Jensen (1984) Biochemistry 23:6619.
4. Wallis, R. et al. (2007) J. Biol. Chem. 282:7844.
5. Murray, L.J. et al. (1999) Exp. Hematol. 27:1282.
6. Haregewoin, A. et al. (1994) Cell. Immunol. 156:357.
7. Hasegawa, M. et al. (2007) Pathol. Int. 57:245.
8. Brashem-Stein, C. et al. (1988) J. Immunol. 140:2330.
9. Hashimoto, M. et al. (2004) Oncogene 23:3716.
10. Solomon, K.R. et al. (2004) Gene 327:171.
11. Finnson, K.W. et al. (2006) FASEB J. 20:1525.
12. Schuh, A.C. et al. (2002) Blood 99:1692.
13. Kelton, J.G. et al. (1998) J. Lab. Clin. Med. 132:142.
14. Rozman, P. (2002) Transpl. Immunol. 10:165.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD109 Antibody (FAB4385P)(3)

Publications using FAB4385P

• Sousa, NS;Bica, M;Brás, MF;Sousa, AC;Antunes, IB;Encarnação, IA;Costa, TM;Martins, IB;Barbosa-Morais, NL;Sousa-Victor, P;Neves, J; The immune landscape of murine skeletal muscle regeneration and aging Cell reports 2024-11-13 [PMID: 39541212] (Flow Cytometry, Mouse, Transgenic Mouse)
• M Minata, A Audia, J Shi, S Lu, J Bernstock, MS Pavlyukov, A Das, SH Kim, YJ Shin, Y Lee, H Koo, K Snigdha, I Waghmare, X Guo, A Mohyeldin, D Gallego-Pe, J Wang, D Chen, P Cheng, F Mukheef, M Contreras, JF Reyes, B Vaillant, EP Sulman, SY Cheng, JM Markert, BA Tannous, X Lu, M Kango-Sing, LJ Lee, DH Nam, I Nakano, KP Bhat Phenotypic Plasticity of Invasive Edge Glioma Stem-like Cells in Response to Ionizing Radiation Cell Rep, 2019-02-12;26(7):1893-1905.e7. 2019-02-12 [PMID: 30759398] (Flow Cytometry, Human)
• D Yamakawa, W Jia, H Kidoya, S Hosojima, M Torigata, L Zhang, N Takakura Visualization of Proliferative Vascular Endothelial Cells in Tumors in�Vivo by Imaging Their Partner of Sld5-1 Promoter Activity Am. J. Pathol., 2018-04-09;0(0):. 2018-04-09 [PMID: 29650228] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: CD109
• Uniprot:
  • Q6YHK3()