CCR5 Antibody

Product Discontinued

CCR5 Antibody Summary

• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human CCR5.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CCR5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/ml
  • Immunohistochemistry 0.5-1 ug/ml
  • Immunohistochemistry-Paraffin 0.5-1 ug/ml
  • Western Blot 0.1-0.5 ug/ml
• Application Notes: Flow Cytometry reactivity reported in scientific literature (PMID: 24166712).
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature [PMID: 24166712]

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, and 0.2mg Na2HPO4
• Preservative: 0.05mg Sodium Azide
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with 0.2 ml of distilled water will yield a concentration of 0.5 mg/ml.

Alternate Names for CCR5 Antibody

• C-C CKR-5
• C-C motif chemokine receptor 5 A159A
• CCCKR5
• CC-CKR-5FLJ78003
• CCR5
• CCR-5
• CD195 antigen
• CD195
• chemokine (C-C motif) receptor 5
• chemokine receptor CCR5
• chemr13
• CKR5
• CKR-5
• HIV-1 fusion coreceptor
• IDDM22CMKBR5C-C chemokine receptor type 5

Background

The CC chemokine receptor 5 (CCR5) is a member of the CC-chemokine receptor family, and has the characteristic structure of a 7 transmembrane G protein-coupled receptor (GPCR). CCR5 regulates trafficking and effector functions of memory/effector Th1 cells, macrophages, NK cells, and immature dendritic cells. CCR5 and its ligands play an important role in viral pathogenesis. CCR5 represents the co-receptor for macrophage (M) and dual (T cell and M)-tropic immunodeficiency viruses. Together with the CD4 binding receptor, CCR5 plays a critical role in HIV entry into the target cells. Moreover, the CCR5 ligands macrophage inflammatory protein (MIP)-1 alpha, MIP-1 beta and RANTES act as endogenous inhibitors of HIV infection, making both CCR5 and its chemokine ligands attractive therapeutic targets for HIV infection. Recent studies have also highlighted the role of CCR5 in a variety of other human diseases, ranging from infectious and inflammatory diseases to cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for CCR5 Antibody (NBP1-41434)(4)

Publications using NBP1-41434

• Ciechanowska A, Popiolek-Barczyk K, Pawlik K et al. Changes in macrophage inflammatory protein-1 (MIP-1) family members expression induced by traumatic brain injury in mice Immunobiology 2020-02-01 [PMID: 32059938] (WB, Mouse)
• Rudd, JM;Pulavendran, S;Ashar, HK;Ritchey, JW;Snider, TA;Malayer, JR;Marie, M;Chow, VTK;Narasaraju, T; Neutrophils Induce a Novel Chemokine Receptors Repertoire During Influenza Pneumonia Front Cell Infect Microbiol 2019-04-16 [PMID: 31041196] (Mouse)
• Posey KL, Coustry F, Veerisetty AC et al. Chondrocyte-Specific Pathology During Skeletal Growth and Therapeutics in a Murine Model of Pseudoachondroplasia. J Bone Miner Res. 2013-11-06 [PMID: 24194321]
• Baba T, Naka K, Morishita S et al. MIP-1alpha/CCL3-mediated maintenance of leukemia-initiating cells in the initiation process of chronic myeloid leukemia. J Exp Med. 2013-10-28 [PMID: 24166712] (FLOW, Mouse)

Review for CCR5 Antibody (NBP1-41434) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: karen posey IHC-P Mouse 01/08/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: mouse growth plate
• Species: Mouse

FAQs for CCR5 Antibody (NBP1-41434). (Showing 1 - 4 of 4 FAQs).

1. We are planning to perform immunohystochemestry using your anti mouse CCR5 antibody (NBP1-41434). You report application for IHC-Paraffin. Notwithstanding, before order it we need to know if this antiboy also works on IHC-frozen.
   • We have not tested NBP1-41434 on frozen sections, and while we expect it to work, we cannot guarantee it. If you would like to test it you will be eligible for the Innovator's Reward Program.
2. May we ask what shall be used to reconstitute NBP1-41434, and how much shall be added to make the stock solution?
   • We recommend reconstituting in DI Water or PBS to a final concentration of 1 mg/mL so in this case add 100uL of DI Water or PBS and spin down to ensure all the antibody is dissolved in the liquid. You can then use the appropriate dilutions for your assays.
3. I ordered and received the anti-CCR5 antibody (NBP1-41434) for IHC on human biopsy. I see in your recommendation that you suggest 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4 as buffer. The histology core here is asking if there are other buffers that are adequate? For the aliquots, is there an optimal concentration for storage? (without BSA?) and or that have been tested. Would PBS be suitable?
   • 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4 is the buffer this product is supplied in its lyophilized form. We recommend reconstituting in100ul of DEPC or mili-Q water and storing in 10ul aliquots.
4. Is the epitope extracellular or intracellular?
   • NBP1-41434 is made to a region within the N-terminus of human CCR5. The immunogen is extracellular.

Bioinformatics

• Gene Symbol: CCR5
• Entrez:
  • 1234(Human)
  • 12774(Mouse)
  • 117029(Rat)
• Uniprot:
  • P51681()