Western Blot: CCR5 Antibody [NB100-531] - WB Image of CCR5 in human liver lysate.
Immunocytochemistry/ Immunofluorescence: CCR5 Antibody [NB100-531] - CCR5 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).
Flow Cytometry: CCR5 Antibody [NB100-531] - Analysis of surface CCR5 expression on CEM.NKR.CCR5 cells. Cells were incubated with 25 ug/ml and were detected with a FITC-labeled goat anti-rabbit IgG. Flow cytometry ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Immunogen affinity purified
Western Blot 1.0 ug/ml
ELISA 1 ug/ml
Flow Cytometry 25 ug/ml
In vitro assay
This CCR5 antibody may be used for Western blot, ELISA, Immunocytochemistry/Immunofluorescence and Flow Cytometry, as well as being useful for inhibition of CCR5 tropic HIV envelope mediated fusion, syncytia formation, and infection in various in vitro assays.
Read Publication using NB100-531 in the following applications:
The CC chemokine receptor 5 (CCR5) is a member of the CC-chemokine receptor family, and has the characteristic structure of a 7 transmembrane G protein-coupled receptor (GPCR). CCR5 regulates trafficking and effector functions of memory/effector Th1 cells, macrophages, NK cells, and immature dendritic cells. CCR5 and its ligands play an important role in viral pathogenesis. CCR5 represents the co-receptor for macrophage (M) and dual (T cell and M)-tropic immunodeficiency viruses. Together with the CD4 binding receptor, CCR5 plays a critical role in HIV entry into the target cells. Moreover, the CCR5 ligands macrophage inflammatory protein (MIP)-1 alpha, MIP-1 beta and RANTES act as endogenous inhibitors of HIV infection, making both CCR5 and its chemokine ligands attractive therapeutic targets for HIV infection. Recent studies have also highlighted the role of CCR5 in a variety of other human diseases, ranging from infectious and inflammatory diseases to cancer.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for CCR5 Antibody (NB100-531). (Showing 1 - 1 of 1 FAQ).
Which non-human primate species has NB100-531 been tested and in what assays? I checked homology with both cyno and rhesus, but the homology with those did not look good. Do you have any info available?
The synthetic peptide used was made to the epitope binding site of the monoclonal antibody 2D7-2SK. The sequence EWQKEGLVTLWLGGGGSC is a combination of the two distal ends of extracellular loop 2 (ECL-2) on the human CCR5 sequence. The binding site is conformation dependent and will only detect the protein if the disulfide bridge between extracellular loop 1 and 2 is intact. It will also not detect the protein in its reduced form. Due to this, we will be removing western blot from our list of viable application. Here is the paper published in the Journal of Virology by the inventor of this antibody. I aligned one of the distal end sequences with human and Rhesus. If you are looking for a CCR5 that will detect primate (Macaque) protein in western blot, I would suggest one of the following: catalog number NB100-714.