CCR5 Antibody (45523.111)

Product Discontinued

CCR5 Antibody (45523.111) Summary

• Immunogen: Human CCR5 transfected NSO mouse myeloma cells.
• Localization: Membrane; multi pass membrane protein
• Specificity: The antibody reacts with human CCR-5 transfectant cells and not the parent cell line. The antibody shows no cross-reactivity with CCR-1, CCR-2 or CCR-3. This antibody can be used to detect CCR-5 present on stimulated human PBMCs that have been fixed with 2% formaldehyde, but not on unfixed cells.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCR5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
• Application Notes: Flow Cytometry Determined using 10 ul human CCR5 transfected cells (1-2.5 x 10⁵ cells) in a toatal reaction volume not exceeding 200 ul. IHC: Use at a concentration of 5 ug/ml for 2% formaldehyde fixed cells.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 5% Trehalose
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for CCR5 Antibody (45523.111)

• C-C CKR-5
• C-C motif chemokine receptor 5 A159A
• CCCKR5
• CC-CKR-5FLJ78003
• CCR5
• CCR-5
• CD195 antigen
• CD195
• chemokine (C-C motif) receptor 5
• chemokine receptor CCR5
• chemr13
• CKR5
• CKR-5
• HIV-1 fusion coreceptor
• IDDM22CMKBR5C-C chemokine receptor type 5

Background

Chemokines have been sub-divided into families on the basis of the relative position of their cysteine residues. The alpha and beta families, with four cysteine residues, are the largest and best characterized. In the alpha family, one amino acid separates the first two cysteine residues (CXC); in the beta family the two cysteine residues (CC) are adjacent to each other. The alpha chemokines that contain the N-terminal Glu-Leu-Arg amino acid sequence (ELR-motif) are chemotactic for neutrophils (such as IL8), while those that do not, act on lymphocytes (such as IP10 and MIG). Examples of chemokines under the beta family category are MCP1-5 and RANTES. The chemokine lymphotactin belongs to the gamma family, with only two cysteines (C), and the recently described fractalkine or neurotactin is a member of the delta family and has the first two cysteine residues separated by three amino-acids (CXXXC). Chemokines bind to specific G protein-coupled cell surface receptors on target cells. Five CXC receptors (CXCR1-5), nine CC receptors (CCR1-9) and one CXXXC receptor (CX₃CR1) have been cloned to date. Expression of chemokine receptors can be restricted to some cell types (CXCR1 is expressed in neutrophils) while others (such as CCR2) are expressed in a wide variety of cells. Receptor expression has also been found to be constitutive (including down regulation), inducible or restricted to a cell state of activation. In addition, some chemokine receptors are also expressed in non-hematopoietic cells, such as nerve, endothelial and epithelial cells. This suggests that chemokines have other roles besides leucocyte chemotaxis. CX₃CR1, for example, is highly expressed in adult brain. Chemokine receptors are linked to phospholipases through the Gi class of G proteins (inhibition by pertussis toxin). Receptor activation leads to a cascade of cellular events including generation of inositol triphosphate, calcium release and activation of protein kinase C. Chemokine receptors also activate small GTP-binding proteins of the Ras and Rho families, the latter being involved in cell motility events. In addition, chemokines bind to non-signaling molecules such as the Duffy antigen receptor for chemokines (DARC) which may act to remove chemokines from the circulation, and heparan sulfates proteoglycans which may serve to establish an ECM concentration gradient. CCR5 has 48 - 75% amino acid sequence identity to earlier identified CC receptors. It is expressed in primary adherent monocytes, but not in neutrophils or eosinophils. CCR5 mediates the activities of MIP1a, MIP1b and RANTES. Recently, it has also been shown to be a co-receptor on CD4+ target cells for infection with primary, monocyte-tropic HIV1 viruses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCR5
• Entrez:
  • 1234(Human)