CCR11 Antibody (674144) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with either (A) human CCR11 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human CCR11 Monoclonal Antibody (Catalog # MAB6899) followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0117). Quadrant markers were set based on control antibody staining . View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 674144
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CCR11 Antibody (674144) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human CCR11
  Accession # Q9NPB9
• Specificity: Detects human CCR11 in direct ELISAs.
• Source: N/A
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCRL1
• Purity Statement: IgM-specific Affinity-purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR11 Antibody (674144) [Unconjugated]

• ACKR4
• CC chemokine receptor-like 1
• C-C CKR-11
• CCBP2CCX CKR
• CC-CKR-11
• CCR11
• CCR11CCR10
• CCRL1
• CCX-CKR
• chemokine (C-C motif) receptor-like 1
• orphan seven-transmembrane receptor, chemokine related
• PPR1
• PPR1cc motif, receptor-like protein 1
• VSHK1
• VSHK1CKR-11

Background

CCR11, also known as CCRL1 and CCX-CKR, is a 45 kDa 7-transmembrane segment protein that is widely expressed on stromal cells. It is a silent receptor that does not induce intracellular calcium signaling, but instead serves as a chemokine scavenger receptor. CCR11 regulates dendritic cell and thymic precursor migration by mediating the clearance of the homeostatic chemokines CCL19, 21, and 25. It is upregulated on some breast cancer cells and on bronchial epithelial cells in pulmonary sarcoidosis. Human CCR11 shares 85% amino acid sequence identity with mouse and rat CCR11.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCRL1
• Uniprot:
  • Q9NPB9()