CCL9/10/MIP-1 gamma Antibody [Unconjugated]

Recombinant Mouse CCL9/10/MIP‑1 gamma (Catalog # 463‑MG) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL9/10/MIP‑1 gamma (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL9/10/MIP‑1 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF463). The ND50 is typically 0.3-1.0 µg/mL.

• Reactivity: Mu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CCL9/10/MIP-1 gamma Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse CCL9/10/MIP‑1 gamma
  Gln22-Gln122
  Accession # Q3U9T8
• Specificity: Detects CCL9/10/MIP‑1 gamma in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant mouse C10 is observed and less than 1% cross-reactivity with recombinant human MPIF-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Ccl9
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.3-1.0 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL9/10/MIP-1 gamma Antibody [Unconjugated]

• C-C motif chemokine 9
• CCF18
• CCL9/10
• chemokine (C-C motif) ligand 9
• macrophage inflammatory protein 1-gamma
• macrophage inflammatory protein-related protein 2
• MIP1 gamma
• MIP-1 gamma
• MRP-2
• SCYA10
• Scya9
• small inducible cytokine A10
• small inducible cytokine A9

Background

Mouse CCL9/10 (also named MIP-1 gamma and MRP-2) is an 11 kDa, secreted, monomeric polypeptide that belongs to the beta (or CC) intercrine family of chemokines (1‑3). Based on its activity and amino acid (aa) sequence, it is further classified as a member of the NC6 or six cysteine-containing CC subfamily of chemokines (2, 4, 5). This subfamily contains four N-terminally extended chemokines, two human (CCL15 and CCL23) and two mouse (CCL9 and CCL10). Within this subfamily, there are no human-to-rodent interspecies orthologs. Mouse CCL9/10 is synthesized as a 122 aa precursor that contains a 21 aa signal sequence and a 101 aa mature region with six cysteines. As noted, the mature region has an expanded N-terminus relative to other CC family members, and it forms a third intrachain disulfide bond with its two extra cysteines (3‑7). Mouse CCL9/10 is 75% aa identical to rat CCL9/10 (8). Chemokines are known to undergo proteolytic processing to generate multiple isoforms. NC6 chemokines are usually only marginally active at full length, but are converted to highly active forms upon N-terminal truncation. Mature CCL9, in the presence of inflammatory fluids, is naturally truncated by 28, 29 or 30 aa at the N-terminus, generating a highly active, 8 kDa, 71‑73 aa CCR1 ligand. In contrast, other CCR1 ligands, CCL3/MIP-1 alpha and CCL5/RANTES, lose their potency when proteolytically processed. CCL9/10 is constitutively secreted, and circulates as a full‑length molecule. Any onset of inflammation with subsequent enzyme release may act on local NC6 chemokines, generating early, potent leukocyte chemoattractants (5, 7).

1. Zlotnik, A. and O. Yoshie (2000) Immunity 12:121.
2. Zlotnik, A. et al. (1999) Crit. Rev. Immunol. 19:1
3. Mohamadzadeh, M. et al. (1996) J. Immunol. 156:3102.
4. Haelens, A. et al. (1996) Immunobiology 195:499.
5. Berahovich, R.D. et al. (2005) J. Immunol. 174:7341.
6. Youn, B-S. et al. (1995) J. Immunol. 155:2661.
7. Poltorak, A.N. et al. (1995) J. Inflamm. 45:207.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL9/10/MIP-1 gamma Antibody (AF463)(6)

Publications using AF463

• Cheung, EC;Strathdee, D;Stevenson, D;Coomes, J;Blyth, K;Vousden, KH; Regulation of ROS signaling by TIGAR induces cancer-modulating responses in the tumor microenvironment Proceedings of the National Academy of Sciences of the United States of America 2024-12-10 [PMID: 39636862] (Immunohistochemistry, Neutralization, Mouse)
• Ciechanowska, A;Pawlik, K;Ciapa?a, K;Mika, J; Pharmacological Modulation of the MIP-1 Family and Their Receptors Reduces Neuropathic Pain Symptoms and Influences Morphine Analgesia: Evidence from a Mouse Model Brain sciences 2023-03-29 [PMID: 37190544] (In Vivo, Mouse)
• Y Sakuma, Y Kodama, T Eguchi, N Uza, Y Tsuji, M Shiokawa, T Maruno, K Kuriyama, Y Nishikawa, Y Yamauchi, M Tsuda, T Ueda, T Matsumori, T Morita, T Tomono, N Kakiuchi, A Mima, Y Sogabe, S Marui, T Kuwada, A Okada, T Watanabe, H Nakase, T Chiba, H Seno Chemokine CXCL16 mediates acinar cell necrosis in cerulein induced acute pancreatitis in mice Sci Rep, 2018-06-11;8(1):8829. 2018-06-11 [PMID: 29891873] (IHC-Fr, Mouse)
• Kortlever Roderik M, Sodir Nicole M, Wilson Catherine H et al. Myc CoopeRates with Ras by Programming Inflammation and Immune Suppression. Cell 1/1/2017 [PMID: 29195074]
• Mishra , Pramod K, Teale , Judy M Changes in gene expression of pial vessels of the blood brain barrier during murine neurocysticercosis. PLoS Negl Trop Dis, 2013-03-14;7(3):e2099. 2013-03-14 [PMID: 23516647] (IHC, Mouse)
• Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D Comprehensive assessment of chemokine expression profiles by flow cytometry. J. Clin. Invest., 2010-02-08;120(3):907-23. 2010-02-08 [PMID: 20197626] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: Ccl9
• Uniprot:
  • Q3U9T8()