CCL8/MCP-2 Antibody (146123) [Unconjugated]

• Reactivity: Mu
• Applications: WB
• Clone: 146123
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

CCL8/MCP-2 Antibody (146123) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse CCL8/MCP-2
  Glu20-Pro97
  Accession # Q9Z121
• Specificity: Detects mouse CCL8/MCP-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant cotton rat CCL3, 4, 5, recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, recombinant mouse CCL1, 3, 4, 5, 6, 7, 9/10MIP-1 gamma , 11, 12, 17, 19, 20, 21, 22, 24, 25, 27, or recombinant rat CCL20 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CCL8
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL8/MCP-2 Antibody (146123) [Unconjugated]

• C-C motif chemokine 8
• CCL8
• chemokine (C-C motif) ligand 8
• HC14
• MCP2
• MCP-2
• member 8 (monocyte chemotactic protein 2)
• monocyte chemoattractant protein 2
• SCYA10
• small-inducible cytokine A8

Background

CCL8, also known as Monocyte Chemoattractant Protein 2 (MCP-2), is an inflammatory CC chemokine that attracts monocytes, eosinophils and basophils. It is produced by many cell types and signals through interactions with CCR1, CCR2, CCR3 and CCR5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL8/MCP-2 Antibody (MAB790)(3)

Publications using MAB790

• L Hu, Y Hayashi, H Kidoya, N Takakura Endothelial cell-derived Apelin inhibits tumor growth by altering immune cell localization Scientific Reports, 2021-07-07;11(1):14047. 2021-07-07 [PMID: 34234274] (IHC, Mouse)
• Dulek D, Newcomb D, Goleniewska K, Cephus J, Zhou W, Reiss S, Toki S, Ye F, Zaynagetdinov R, Sherrill T, Blackwell T, Moore M, Boyd K, Kolls J, Peebles R Allergic airway inflammation decreases lung bacterial burden following acute Klebsiella pneumoniae infection in a neutrophil- and CCL8-dependent manner. Infect Immun, 2014-06-23;82(9):3723-39. 2014-06-23 [PMID: 24958709] (Neutralization, Mouse)
• Di Valentin E, Crahay C, Garbacki N, Hennuy B, Gueders M, Noel A, Foidart JM, Grooten J, Colige A, Piette J, Cataldo D New asthma biomarkers: lessons from murine models of acute and chronic asthma. Am. J. Physiol. Lung Cell Mol. Physiol., 2008-11-21;296(2):L185-97. 2008-11-21 [PMID: 19028979] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: CCL8
• Uniprot:
  • Q9Z121()