CCL8/MCP-2 Antibody (146123) [Janelia Fluor® 525]

• Reactivity: Mu
• Applications: WB
• Clone: 146123
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Janelia Fluor 525

CCL8/MCP-2 Antibody (146123) [Janelia Fluor® 525] Summary

• Immunogen: E. coli-derived recombinant mouse CCL8/MCP-2
  Glu20-Pro97
  Accession # Q9Z121
• Specificity: Detects mouse CCL8/MCP-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant cotton rat CCL3, 4, 5, recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, recombinant mouse CCL1, 3, 4, 5, 6, 7, 9/10MIP-1 gamma , 11, 12, 17, 19, 20, 21, 22, 24, 25, 27, or recombinant rat CCL20 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CCL8
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for CCL8/MCP-2 Antibody (146123) [Janelia Fluor® 525]

• C-C motif chemokine 8
• CCL8
• chemokine (C-C motif) ligand 8
• HC14
• MCP2
• MCP-2
• member 8 (monocyte chemotactic protein 2)
• monocyte chemoattractant protein 2
• SCYA10
• small-inducible cytokine A8

Background

MCP2 and MCP3 are two recently identified monocyte chemotactic proteins produced by human MG63 osteosarcoma cells. Both MCP2 and MCP3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP1. MCP3 also shares 58% amino acid identity with MCP2. Similarly to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCL8