CCL7/MCP-3/MARC Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CCL7/MCP-3/MARC Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human CCL7/MCP-3 (R&D Systems, Catalog # 282-P3)
  Gln34-Leu109
  Accession # Q7Z7Q8
• Specificity: Detects human CCL7/MCP-3 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) Eotaxin, recombinant mouse (rm) JE, rhI-309, rhMCP-1, rhMCP-2, rmMCP-3, rhMCP-4, and rmMCP-5 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCL7
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human CCL7/MCP-3/MARC Antibody (Catalog # MAB282)
  ELISA Detection: Human CCL7/MCP-3/MARC Biotinylated Antibody (Catalog # BAF282)
  Standard: Recombinant Human CCL7/MCP-3/MARC (Catalog # 282-P3)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL7/MCP-3/MARC Antibody [Biotin]

• C-C motif chemokine 7
• CCL7
• chemokine (C-C motif) ligand 7
• FIC
• MARC
• MCP-3
• MCP3Monocyte chemotactic protein 3
• MCP-3Small-inducible cytokine A7
• MGC138465
• Monocyte chemoattractant protein 3
• NC28SCYA6
• SCYA7MGC138463
• small inducible cytokine A7 (monocyte chemotactic protein 3)

Background

MCP-2 and CCL7 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and CCL7 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. CCL7 also shares 58% amino acid identity with MCP-2.

CCL7 cDNA encodes a 99 amino acid residue precursor protein from which the N-terminal 23 amino acid residues are cleaved to generate the 76 amino acid residue mature CCL7. Mature CCL7 contains a potential N-linked and several possible O-linked glycosylation sites.

Similarly to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4⁺ and CD8⁺ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL7/MCP-3/MARC Antibody (BAF282)(1)

Publication using BAF282

• Mehdi Bouhaddou, Danish Memon, Bjoern Meyer, Kris M. White, Veronica V. Rezelj, Miguel Correa Marrero et al. The Global Phosphorylation Landscape of SARS-CoV-2 Infection Cell 2020-08-01 [PMID: 32645325]

Bioinformatics

• Gene Symbol: CCL7
• Uniprot:
  • Q7Z7Q8()