CCL3/CCL4 Antibody (93342) [Fluorescein]

Human peripheral blood monocytes treated with LPS were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCL3/CCL4 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC2701F) or (B) Mouse IgG2BFluorescein Isotype Control (Catalog # IC0041F). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 93342
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein

CCL3/CCL4 Antibody (93342) [Fluorescein] Summary

• Immunogen: E. coli-derived recombinant human CCL3/MIP-1 alpha
  Ala27-Ala92
  Accession # P10147
• Specificity: Detects human CCL3/MIP-1 alpha in Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) CCL4/MIP-1 beta is observed. Does not cross-react with rhCCL1, 2, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, recombinant mouse CCL1, 2, 3, 5, 6, 7, CCL9/10MIP-1 gamma , 11, 12, 17, 19, 20, 21, 22, 24, 25, or recombinant rat CCL20.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCL3
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL3/CCL4 Antibody (93342) [Fluorescein]

• C-C motif chemokine;C-C motif chemokine 3;G0S19-1;LD78ALPHA;MIP1A;MIP-1-alpha;SCYA3
• CCL3/CCL4

Background

The Macrophage Inflammatory Proteins -1 alpha and -1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP-1 alpha refers to the products of several independently cloned cDNAs, including LD78, pL78, pAT464, and GOS19. These cDNAs all code for the same human protein that is a homologue of the murine MIP-1 alpha . Mature MIP-1 alpha and MIP-1 beta in both human and mouse share approximately 70% homology at the amino acid level. The MIP‑1 proteins are members of the beta (C-C) subfamily of chemokines.

Both MIP-1 alpha and MIP-1 beta are monocyte chemoattractants in vitro. Additionally, the MIP-1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP-1 alpha and MIP-1 beta preferentially attracting CD8⁺ and CD4⁺ T cells, respectively. MIP-1 alpha has also been shown to attract B cells as well as eosinophils. MIP-1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP-1 alpha has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. The functional receptor for MIP-1 alpha has been identified as CCR1 and CCR5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL3/CCL4 Antibody (IC2701F)(2)

Publications using IC2701F

• Hong M, Sandalova E, Low D, Gehring A, Fieni S, Amadei B, Urbani S, Chong Y, Guccione E, Bertoletti A Trained immunity in newborn infants of HBV-infected mothers. Nat Commun, 2015-03-25;6(0):6588. 2015-03-25 [PMID: 25807344] (Flow Cytometry, IHC-Fr, Human)
• Hutnick NA, Carnathan D, Demers K, Makedonas G, Ertl HC, Betts MR Adenovirus-specific human T cells are pervasive, polyfunctional, and cross-reactive. Vaccine, 2010-02-23;28(8):1932-41. 2010-02-23 [PMID: 20188249] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CCL3
• Uniprot:
  • P10147()