CCL27/CTACK Antibody

Recombinant Human CCL27/CTACK (Catalog # 376‑CT) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR10/GPR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL27/CTACK (1 µg/mL) is neutralized (green line) by increasing concentrations of Human CCL27/CTACK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF376). At 55 μg/mL, this antibody will neutralize > 70% of the chemotactic effect.

CCL27/CTACK was detected in immersion fixed frozen sections of human skin using Human CCL27/CTACK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF376) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hemotoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CCL27/CTACK Antibody Summary

• Immunogen: E. coli-derived recombinant human CCL27/CTACK (R&D Systems, Catalog # 376-CT)
  Phe25-Gly112
  Accession # Q9Y4X3
• Specificity: Detects human CCL27/CTACK in direct ELISAs and Western blots. In Western blots, less than 2% cross-reactivity with recombinant mouse CCL27/CTACK is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCL27
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL27/CTACK Antibody

• ALP
• C-C motif chemokine 27
• CCL27
• chemokine (C-C motif) ligand 27
• CTACK
• CTACKSmall-inducible cytokine A27
• CTAK
• cutaneous T-cell attracting chemokine
• Cutaneous T-cell-attracting chemokine
• ESKINE
• IL-11 Ralpha-locus chemokine
• ILC
• ILCCC chemokine ILC
• PESKY
• SCYA27IL-11 R-alpha-locus chemokine
• skinkine
• small inducible cytokine subfamily A (Cys-Cys), member 27

Background

CCL27, also known as CTACK (cutaneous T cell-attracting chemokine), ALP, ILC, and ESkine, is a member of the CC family of chemokines. Mature human CCL27 is an 88 amino acid (aa) protein that shares 57% aa sequence identity with mouse and rat CCL27. It shares 11-35% aa sequence identity with other human CC chemokines. An alternately spliced form of mouse CCL27, known as PESKY, is localized to the nucleus and promotes cellular migration. CCL27 is constitutively expressed by keratinocytes and is upregulated by inflammatory stimuli and in wounded skin. CCL27 binds the chemokine receptor CCR10, glycosaminoglycans in the extracellular matrix, sulfated tyrosine residues on PSGL-1, and determinants on the surface of fibroblasts and endothelial cells. CCL27 cooperates with CCL17/TARC in inducing the migration of cutaneous lymphocyte antigen (CLA) positive memory T cells to the skin during inflammation. Endothelial cell-bound CCL27 can mediate the adhesion of those cells to CLA⁺ T cells. CCL27 also induces the migration of keratinocyte precursors from bone marrow to the skin, thereby promoting wound healing. In humans, serum CCL27 levels are elevated and correlate with disease severity in atopic dermatitis, psoriasis vulgaris, and mycosis fungoides.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL27/CTACK Antibody (AF376)(2)

Publications using AF376

• MJ Mao, N Xue, XP Wang, PD Chi, YJ Liu, Q Huang, SQ Dai, WL Liu Chemokine CCL27 is a novel plasma biomarker for identification the nasopharyngeal carcinoma patients from the Epstein-Barr virus capsid antigen-specific IgA seropositive population BMC Cancer, 2018-01-02;18(1):9. 2018-01-02 [PMID: 29295705] (IHC, Human)
• Jaeger J, Koczan D, Thiesen HJ, Ibrahim SM, Gross G, Spang R, Kunz M Gene expression signatures for tumor progression, tumor subtype, and tumor thickness in laser-microdissected melanoma tissues. Clin. Cancer Res., 2007-02-01;13(3):806-15. 2007-02-01 [PMID: 17289871] (IHC-Fr, IHC-P, Western Blot, Human)

Bioinformatics

• Gene Symbol: CCL27
• Uniprot:
  • Q9Y4X3()