CCL27/CTACK Antibody (124302)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, ELISA(Cap)
Clone
124302
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

CCL27/CTACK Antibody (124302) Summary

Immunogen
E. coli-derived recombinant human CCL27/CTACK
Phe25-Gly112
Accession # NP_006655
Specificity
Detects human CCL27/CTACK in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant mouse CCL27, recombinant human (rh) CCL25, or rhCCL19 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CCL27
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Detection: Human CCL27/CTACK Biotinylated Antibody (Catalog number BAF376)
Standard: Recombinant Human CCL27/CTACK (Catalog number 376-CT)
Publications
Read Publications using
MAB3761 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL27/CTACK Antibody (124302)

  • ALP
  • C-C motif chemokine 27
  • CCL27
  • chemokine (C-C motif) ligand 27
  • CTACK
  • CTACKSmall-inducible cytokine A27
  • CTAK
  • cutaneous T-cell attracting chemokine
  • Cutaneous T-cell-attracting chemokine
  • ESKINE
  • IL-11 Ralpha-locus chemokine
  • ILC
  • ILCCC chemokine ILC
  • PESKY
  • SCYA27IL-11 R-alpha-locus chemokine
  • skinkine
  • small inducible cytokine subfamily A (Cys-Cys), member 27

Background

CCL27, also known as CTACK (cutaneous T cell-attracting chemokine), ALP, ILC, and ESkine, is a member of the CC family of chemokines (1). Mature human CCL27 is an 88 amino acid (aa) protein that shares 57% aa sequence identity with mouse and rat CCL27 (2). It shares 11%‑35% aa sequence identity with other human CC chemokines. An alternately spliced form of mouse CCL27, known as PESKY, is localized to the nucleus and promotes cellular migration (3). CCL27 is constitutively expressed by keratinocytes and is up‑regulated by inflammatory stimuli and in wounded skin (4‑7). CCL27 binds the chemokine receptor CCR10, glycosaminoglycans in the extracellular matrix, sulfated tyrosine residues on PSGL-1, and determinants on the surface of fibroblasts and endothelial cells (5, 7‑9). CCL27 cooperates with CCL17/TARC in inducing the migration of cutaneous lymphocyte antigen (CLA) positive memory T cells to the skin during inflammation (4, 6, 10‑12). Endothelial cell-bound CCL27 can mediate the adhesion of those cells to CLA+ T cells (6). CCL27 also induces the migration of keratinocyte precursors from bone marrow to the skin, thereby promoting wound healing (7). In humans, serum CCL27 levels are elevated and correlate with disease severity in atopic dermatitis, psoriasis vulgaris, and mycosis fungoides (13‑15).

  1. Kunkel, L. and E.C. Butcher (2002) Immunity 16:1.
  2. Ishikawa-Mochizuki, I. et al. (1999) FEBS Lett. 460:544.
  3. Gortz, A. et al. (2002) J. Immunol. 169:1387.
  4. Morales, J. et al. (1999) Proc. Natl. Acad. Sci. 96:14470.
  5. Homey, B. et al. (2000) J. Immunol. 164:3465.
  6. Homey, B. et al. (2002) Nat. Med. 8:157.
  7. Inokuma, D. et al. (2006) Stem Cells 24:2810.
  8. Jarmin, D. et al. (2000) J. Immunol. 164:3460.
  9. Hirata, T. et al. (2004) J. Biol. Chem. 279:51775.
  10. Vestergaard, C. et al. (2004) Exp. Dermatol. 13:551.
  11. Reiss, Y. et al. (2001) J. Exp. Med. 194:1541.
  12. Soler, D. et al. (2003) Blood 101:1677.
  13. Kakinuma, T. et al. (2003) J. Allergy Clin. Immunol. 111:592.
  14. Hijnen, D. et al. (2004) J. Allergy Clin. Immunol. 113:334.
  15. Fujita, Y. et al. (2006) Clin. Cancer Res. 12:2670.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL27/CTACK Antibody (MAB3761)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC Fresh/Frozen, IHC Frozen.


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IHC Fresh/Frozen
(1)
IHC Frozen
(2)
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(4)
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Bioinformatics

Gene Symbol CCL27
Entrez
Uniprot