CCL24/Eotaxin-2/MPIF-2 Antibody (61016) [Unconjugated]

Recombinant Human CCL24/Eotaxin‑2 (Catalog # 343‑E2) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL24/Eotaxin‑2 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL24/Eotaxin‑2 Monoclonal Antibody (Catalog # MAB343). The ND50 is typically 0.2‑0.8 µg/mL.

• Reactivity: Hu
• Applications: WB, B/N, ELISA(Cap)
• Clone: 61016
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CCL24/Eotaxin-2/MPIF-2 Antibody (61016) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CCL24/Eotaxin‑2/MPIF‑2
  Val27-Cys119
  Accession # AAB51135
• Specificity: Detects human CCL24/Eotaxin‑2/MPIF‑2 in ELISAs and Western blots. In sandwich ELISAs, less than 0.04% cross-reactivity with recombinant human (rh) Eotaxin, rhMIP-1 alpha , and rhMCP-3 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCL24
• Purity Statement: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.2-0.8 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Human CCL24/Eotaxin-2/MPIF-2 Biotinylated Antibody (Catalog number BAF343)
  Standard: Recombinant Human CCL24/Eotaxin-2/MPIF-2 (Catalog number 343-E2)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL24/Eotaxin-2/MPIF-2 Antibody (61016) [Unconjugated]

• CCL24
• chemokine (C-C motif) ligand 24
• Ckb-6
• Eosinophil chemotactic protein 2
• Eotaxin-2
• member 24
• MPIF2
• MPIF-2
• MPIF2Myeloid progenitor inhibitory factor 2
• MPIF-2Small-inducible cytokine A24
• SCYA24CK-beta-6

Background

Eotaxin-2, also named MPIF-2 and Ck beta 6, is a CC chemokine that is designated CCL24. Eotaxin-2 cDNA encodes a 10.5 kDa, 119 amino acid residue precursor protein with a 26 aa residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N-glycosylation site. C‑terminally truncated variants with 78, 73, 75 and 76 residues have also been described. Eotaxin-2 shares 40%, 42% and 39% amino acid sequence identity with other CC chemokines CCL7/MCP-3, CCL3/MIP-1 alpha , and CCL11/Eotaxin, respectively. Eotaxin-2 mRNA is weakly expressed in activated monocytes and T lymphocytes. Recombinant Eotaxin-2 induces chemotaxis of eosinophils, basophils, and resting T lymphocytes, but not monocytes or activated T lymphocytes. Eotaxin-2 also suppresses colony formation by high proliferative multipotential hematopoietic progenitors. On eosinophils, the effects of Eotaxin-2, Eotaxin and CCL13/MCP-4 are mediated by the receptor CCR3.

1. Forssmann, U. et al. (1997) J. Exp. Med. 185:2171.
2. Patel, V.P. et al. (1997) J. Exp. Med. 185:1163.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL24/Eotaxin-2/MPIF-2 Antibody (MAB343)(3)

Publications using MAB343

• JG Li, J Chiu, M Ramanjulu, BE Blass, D Praticò A pharmacological chaperone improves memory by reducing A&amp;beta and tau neuropathology in a mouse model with plaques and tangles Mol Neurodegener, 2020-01-22;15(1):1. 1/22/2020 [PMID: 31964406]
• Jiayin Shen, Yingwen Hu, Karson S. Putt, Sunil Singhal, Haiyong Han, Daniel W. Visscher et al. Assessment of folate receptor alpha and beta expression in selection of lung and pancreatic cancer patients for receptor targeted therapies Oncotarget 1/12/2018 [PMID: 29435118]
• Cheadle EJ, Riyad K, Subar D, Rothwell DG, Ashton G, Batha H, Sherlock DJ, Hawkins RE, Gilham DE Eotaxin-2 and colorectal cancer: a potential target for immune therapy. Clin. Cancer Res., 2007-10-01;13(19):5719-28. 2007-10-01 [PMID: 17908961] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: CCL24
• Uniprot:
  • AAB51135()