CCL22/MDC Antibody (57203) [Unconjugated]

Human mature dendritic cells were stained with Mouse Anti-Human CCL22/MDC Monoclonal Antibody (Catalog # MAB3361, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

• Reactivity: Hu
• Applications: WB, CyTOF-ready, Flow
• Clone: 57203
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CCL22/MDC Antibody (57203) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CCL22/MDC
  Gly25-Gln93
  Accession # O00626.1
• Specificity: Detects human CCL22/MDC in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant cotton rat CCL3, 4, 5, recombinant human CCL1, 2, 3, 4, 5, 7, 8, 9/10/MIP-1 gamma , 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 23, 24, 25, 26, 27, 28, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 8, 9/10/MIP-1 gamma , 11, 12, 17, 19, 21, 22, 24, 25, 27, 28, or recombinant rat CCL20.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCL22
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL22/MDC Antibody (57203) [Unconjugated]

• A-152E5.1
• ABCD-1
• CC chemokine STCP-1
• C-C motif chemokine 22
• CCL22
• chemokine (C-C motif) ligand 22
• DC/B-CK
• Macrophage-derived chemokine
• MDC
• MDCStimulated T-cell chemotactic protein 1
• MGC34554
• SCYA22MDC(1-69)
• small inducible cytokine A22
• small inducible cytokine subfamily A (Cys-Cys), member 22
• Small-inducible cytokine A22
• STCP-1
• stimulated T cell chemotactic protein 1

Background

CCL22, also known as Stimulated T cell Chemotactic Protein (STCP-1), is a CC chemokine initially isolated from clones of monocyte-derived macrophages. Human CCL22 cDNA encodes a precursor protein of 93 amino acid residues with a 24 amino acid residue predicted signal peptide that is cleaved to yield a 69 amino acid residue mature 8 kDa protein. At the amino acid sequence level, CCL22 shows less than 35% identity to other CC chemokine family members. Human CCL22 is expressed in dendritic cells, macrophages and activated monocytes. In addition, CCL22 expression is also detected in the tissues of thymus, lymph node and appendix. The gene for human CCL22 has been mapped to chromosome 16 rather than chromosome 17 where the genes for many human CC chemokines are clustered. Recombinant or chemically synthesized mature CCL22 has been shown to induce chemotaxis or Ca²⁺ mobilization in dendritic cells, IL-2 activated NK cells, and activated T lymphocytes. A CD8⁺ T lymphocyte-derived secreted soluble activity that suppresses infection by primary non-syncytium-inducing and syncytium-inducing HIV-1 isolates and the T-cell line-adapted isolate HIV-1_IIIB, has been identified as CCL22. Based on amino-terminal sequence analysis, the major CD8⁺ T lymphocyte-derived CCL22 protein yielded an amino-terminal sequence of YGANM, which is two amino acid residues shorter than the predicted mature CCL22. The difference in potency between the two mature CCL22 isoforms has not been determined.

1. Godiska, R. et al. (1997) J. Exp. Med. 185:1595.
2. Chang, M-S. et al. (1997) J. Biol. Chem. 272:25229.
3. Pal, R. et al. (1997) Science 278:5338.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCL22
• Uniprot:
  • O00626.1()