CCL21/6Ckine Antibody (709420)

Western blot shows lysates of SVEC4-10 mouse vascular endothelial cell line and D3 mouse embryonic stem cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse CCL21/6Ckine Monoclonal Antibody (Catalog # MAB4571) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CCL21/6Ckine at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Mu
• Applications: WB
• Clone: 709420
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

CCL21/6Ckine Antibody (709420) Summary

• Immunogen: E. coli-derived recombinant mouse CCL21/6Ckine
  Ser24-Gly133
  Accession # P84444
• Specificity: Detects recombinant mouse CCL21/6Ckine in direct ELISAs and mouse CCL21/6Ckine in Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) CCL2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, rhCCL3L1, rhCCL4L1, recombinant mouse (rm) CCL1, 2, 3, 4, 5, 6, 7, 8, 9/10/MIP-1g, 11, 12, 17, 19, 20, 22, 24, 25, 27, 28, rmEotaxin-3-like, recombinant rat (rr) CCL2, 20, rrEotaxin-3-like, recombinant canine CCL2, 3, 4, 5, recombinant cotton rat CCL2, 3, 4, 5, recombinant feline CCL5, or recombinant guinea pig CCL11 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Ccl21a
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL21/6Ckine Antibody (709420)

• 6Ckine
• 6CkineSmall-inducible cytokine A21
• Beta-chemokine exodus-2
• CCL21
• chemokine (C-C motif) ligand 21
• CKb9
• exodus-2
• member 21
• SCYA21
• SCYA21MGC34555
• secondary lymphoid tissue chemokine
• SLC
• SLCSecondary lymphoid-tissue chemokine
• TCA-4

Background

6Ckine is a novel CC chemokine discovered independently by three groups from the EST database. 6Ckine, also named SLC (secondary lymphoid-tissue chemokine), CCL21 and Exodus-2, shows 21‑33% identity to other CC chemokines. 6Ckine contains the four conserved cysteines characteristic of beta chemokines plus two additional cysteines in its unusually long carboxyl-terminal domain. Human 6Ckine cDNA encodes a 134 amino acid highly basic precursor protein with a 23 amino acid residue signal peptide that is cleaved to form the predicted 111 amino acid residue mature protein. Mouse 6Ckine cDNA encodes a 133 amino acid residue protein with a 23 residue signal peptide that is cleaved to generate the 110 residue mature protein. Human and mouse 6Ckine share 86% amino acid sequence identity. 6Ckine is constitutively expressed at high levels in lymphoid tissues such as lymph nodes, spleen and appendix. In mouse, high levels of 6Ckine mRNA are also detected in the lung. Unlike most CC chemokines, 6Ckine is not chemotactic for monocytes. Recombinant mouse 6Ckine is chemotactic in vitro for thymocytes and activated T cells. Recombinant human 6Ckine has been shown to be chemotactic for some human T cell lines, resting PBL, and cultured T cells expanded with PHA and IL-2. 6Ckine has also been reported to inhibit hemopoietic progenitor colony formation in a dose-dependent manner. 6Ckine acts via the CC receptor CCR7 on T cells and B cells.

1. Hedrick, J.A. and A. Zlotnik (1997) J. Immunol. 159:1589.
2. Hromas, R. et al. (1997) J. Immunol. 159:2554.
3. Nagira, M. et al. (1997) J. Biol. Chem. 272:19518.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Ccl21a
• Uniprot:
  • P84444()