CCL21/6Ckine Antibody (709420) [CoraFluor™ 1]

• Reactivity: Mu
• Applications: WB
• Clone: 709420
• Clonality: Monoclonal
• Host: Rat
• Conjugate: CoraFluor 1

CCL21/6Ckine Antibody (709420) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: E. coli-derived recombinant mouse CCL21/6Ckine
  Ser24-Gly133
  Accession # P84444
• Specificity: Detects recombinant mouse CCL21/6Ckine in direct ELISAs and mouse CCL21/6Ckine in Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) CCL2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, rhCCL3L1, rhCCL4L1, recombinant mouse (rm) CCL1, 2, 3, 4, 5, 6, 7, 8, 9/10/MIP-1g, 11, 12, 17, 19, 20, 22, 24, 25, 27, 28, rmEotaxin-3-like, recombinant rat (rr) CCL2, 20, rrEotaxin-3-like, recombinant canine CCL2, 3, 4, 5, recombinant cotton rat CCL2, 3, 4, 5, recombinant feline CCL5, or recombinant guinea pig CCL11 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Ccl21a
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for CCL21/6Ckine Antibody (709420) [CoraFluor™ 1]

• 6Ckine
• 6CkineSmall-inducible cytokine A21
• Beta-chemokine exodus-2
• CCL21
• chemokine (C-C motif) ligand 21
• CKb9
• exodus-2
• member 21
• SCYA21
• SCYA21MGC34555
• secondary lymphoid tissue chemokine
• SLC
• SLCSecondary lymphoid-tissue chemokine
• TCA-4

Background

CCL21 is found on the p-arm of chromosome 9 with other CC cytokine genes. As part of the cytokine family of proteins, CCL21 is involved in immunoregulatory and inflammatory processes. CCL21 is also thought to have a role in mediating homing of lymphocytes to secondary lymphoid organs. This protein is known to have interactions with UBQLN4, VCAN, CCR7, CTSD and CCBP2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Ccl21a