Rat CCL2/MCP1 ELISA Kit (Colorimetric)

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Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA1831
    • Availability
      Product Discontinued

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Rat CCL2/MCP1 ELISA Kit (Colorimetric) Summary

Description
Quality control test: Standard Curve. Ccl2 (Rat) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat Ccl2.
Sensitivity
15 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 12%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CCL2

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
Useful for quantification.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.

Kit Components

Components
  1. Assay Diluent B (Item E): 5x concentrated buffer. For Standard/Sample(cell culture medium) diluent.
  2. Detection Antibody MCP-1 (Item F): biotinylated anti-rat MCP-1 (eachvial is enough to assay half microplate)
  3. HRP-Streptavidin concentrate (Item G): 100x concentratedHRP-conjugated streptavidin.
  4. MCP-1 Microplate (Item A): coated with anti-rat MCP-1.
  5. Standard (Item C): recombinant rat MCP-1.
  6. Stop Solution (Item I): 0.2 M sulfuric acid.
  7. TMB One-Step Substrate Reagent (Item H): 3, 3’, 5, 5’-tetramethylbenzidine (TMB) in buffered solution.
  8. Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Rat CCL2/MCP1 ELISA Kit (Colorimetric)

  • C-C motif chemokine 2
  • chemokine (C-C motif) ligand 2
  • GDCF-2
  • HC11Monocyte secretory protein JE
  • MCAFMonocyte chemotactic and activating factor
  • MCP1 Monocyte chemotactic protein 1
  • MCP-1 Small-inducible cytokine A2
  • MGC9434
  • Monocyte chemoattractant protein 1
  • monocyte chemoattractant protein-1
  • SCYA2HSMCR30
  • small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je)
  • small inducible cytokine subfamily A (Cys-Cys), member 2
  • SMC-CF

Background

Monocyte Chemoattracctant Protein-1 (MCP1), also known as CCL2, is produced by a wide range of cell types as a reaction to diverse inflammatory stimuli and is a chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. MCP1 augments monocyte anti-tumor activity and has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. In atherosclerosis MCP-1 has been implicated to be involved in the recruitment of monocytes into the arterial wall during the disease process.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for CCL2/MCP1 ELISA Kit (KA1831). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Additional CCL2/MCP1 Products

Research Areas for CCL2/MCP1 ELISA Kit (KA1831)

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Blogs on CCL2/MCP1.

MCP-1 - chemoattractant protein involed in monocyte migration and infiltration
Monocyte chemotractant protein-1 (MCP-1), also known as CCL2, is a key chemokine involved in the migration of monocytes and macrophages to sites of active inflammation. It is a member of the C-C/beta family of cytokines, characterized by the Cys-C...  Read full blog post.

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Bioinformatics

Gene Symbol CCL2