Rat CCL2/MCP1 ELISA Kit (Colorimetric) Summary
| Description |
Quality control test: Standard Curve. Ccl2 (Rat) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat Ccl2. |
| Sensitivity |
15 pg/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 12% |
| Intra-Assay |
CV% < 10% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
CCL2 |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Useful for quantification. |
Packaging, Storage & Formulations
| Storage |
Store at -20 to -80C. Avoid freeze-thaw cycles. |
Kit Components
|
Components
|
- Assay Diluent B (Item E): 5x concentrated buffer. For Standard/Sample(cell culture medium) diluent.
- Detection Antibody MCP-1 (Item F): biotinylated anti-rat MCP-1 (eachvial is enough to assay half microplate)
- HRP-Streptavidin concentrate (Item G): 100x concentratedHRP-conjugated streptavidin.
- MCP-1 Microplate (Item A): coated with anti-rat MCP-1.
- Standard (Item C): recombinant rat MCP-1.
- Stop Solution (Item I): 0.2 M sulfuric acid.
- TMB One-Step Substrate Reagent (Item H): 3, 3’, 5, 5’-tetramethylbenzidine (TMB) in buffered solution.
- Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Rat CCL2/MCP1 ELISA Kit (Colorimetric)
Background
Monocyte Chemoattracctant Protein-1 (MCP1), also known as CCL2, is produced by a wide range of cell types as a reaction to diverse inflammatory stimuli and is a chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. MCP1 augments monocyte anti-tumor activity and has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. In atherosclerosis MCP-1 has been implicated to be involved in the recruitment of monocytes into the arterial wall during the disease process.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for CCL2/MCP1 ELISA Kit (KA1831). (Showing 1 - 1 of 1 FAQ).
-
wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.