CCL2/MCP1 Antibody (5D3-F7)

Product Discontinued

CCL2/MCP1 Antibody (5D3-F7) Summary

• Immunogen: Recombinant human MCP-1
• Specificity: Mouse Monoclonal to MCP-1 (5D3-F7)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCL2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume. The purified 5D3-F7 has been tested by blocking fluorochrome conjugated 5D3-F7 for intracellular cytokine staining. In order to obtain complete blocking results, a saturated amount of purified antibody ( le 5.0 ug/million cells) should be used for incubation with target cells, prior to staining with fluorochrome conjugated antibody. Immunohistochemistry-frozen/paraffin. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Expected Cross-Reactivity based on immunogen sequence: Cynomolgus, Rhesus.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for CCL2/MCP1 Antibody (5D3-F7)

• C-C motif chemokine 2
• chemokine (C-C motif) ligand 2
• GDCF-2
• HC11Monocyte secretory protein JE
• MCAFMonocyte chemotactic and activating factor
• MCP1 Monocyte chemotactic protein 1
• MCP-1 Small-inducible cytokine A2
• MGC9434
• Monocyte chemoattractant protein 1
• monocyte chemoattractant protein-1
• SCYA2HSMCR30
• small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je)
• small inducible cytokine subfamily A (Cys-Cys), member 2
• SMC-CF

Background

Monocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 5D3-F7 antibody reacts with human monocyte chemoattractant protein-1 (MCP-1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL2/MCP1 Antibody (NB100-78159)(1)

Publication using NB100-78159

• Gilbert DC, Chandler I, Summersgill B et al. Genomic gain and over expression of CCL2 correlate with vascular invasion in stage I non-seminomatous testicular germ-cell tumours. Int J Androl 2011-08-01 [PMID: 21615418] (Human)

FAQs for CCL2/MCP1 Antibody (NB100-78159). (Showing 1 - 1 of 1 FAQ).

1. I was looking for MCP1 antibody (5D3-F7) which has catalog number NB100-78159. I have surveyed few manuscript which mention the clone number (5D3-F7) but neither the company's name or the catalog number. I would like to whether all those reference indicate your product NB100-78159? We definitely want to purchase that antibody but make sure that is the right antibody.
   • I do not know whether those manuscripts mention our product because I am not familiar with those references. However, if it cites the same clone number for a particular target, it is more than likely the same antibody.

Bioinformatics

• Gene Symbol: CCL2
• Entrez:
  • 6347(Human)