CCL2/JE/MCP-1 Antibody (24822) [Alexa Fluor® 488]

Human peripheral blood mononuclear cells (PBMCs) treated with 250 ng/mL LPS overnight were stained with Mouse Anti-Human CCL2/JE/MCP-1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC279G) and Mouse Anti-Human CD11c APC-conjugated Monoclonal Antibody (Catalog # FAB1777A). Quadrant markers were set based on control antibody staining (Catalog # IC002G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 24822
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

CCL2/JE/MCP-1 Antibody (24822) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human CCL2/JE/MCP-1
  Accession # P13500
• Specificity: Detects human CCL2/JE/MCP-1 in Western blots. Does not cross-react with recombinant human CCL1, 3, 4, 5, 7, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9,10, MIP-1 gamma , 11, 12, 17, 19, 20, 22, 24, 25, or recombinant rat CCL20.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCL2
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL2/JE/MCP-1 Antibody (24822) [Alexa Fluor® 488]

• C-C motif chemokine ligand 2
• CCL2
• GDCF-2
• HC11
• HSMCR30
• MCAF
• Mcp1
• MCP-1
• SCYA2
• SMC-CF

Background

CCL2 is a beta -chemokine that is also known as Monocyte Chemotactic Protein 1 (MCP-1). It is produced by a variety of cell types and is chemotactic for monocytes and basophils. MCP-1 exists in equilibrium as either a monomer or homodimer, and forms a heterodimer with multiple chemokines. Human and mouse CCL2 share 58% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCL2
• Uniprot:
  • P13500()