CCL2/JE/MCP-1 Antibody (23002) [Unconjugated]

CCL2/JE/MCP-1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Mouse Anti-Human CCL2/JE/MCP-1 Monoclonal Antibody (Catalog # MAB2791) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 23002
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CCL2/JE/MCP-1 Antibody (23002) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CCL2/MCP-1
  Gln24-Thr99
  Accession # P13500
• Specificity: Detects human CCL2/JE/MCP-1 in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant human CCL1, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9/10/MIP-1 gamma , 11, 12, 17, 19, 20, 21, 22, 24, 25, or recombinant rat CCL20.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCL2
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL2/JE/MCP-1 Antibody (23002) [Unconjugated]

• C-C motif chemokine ligand 2
• CCL2
• GDCF-2
• HC11
• HSMCR30
• MCAF
• Mcp1
• MCP-1
• SCYA2
• SMC-CF

Background

MCP-1 is a beta -chemokine that has been designated CCL2. It is produced by a variety of cell types and is chemotactic for monocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL2/JE/MCP-1 Antibody (MAB2791)(20)

Publications using MAB2791

• Choi YW, Kim YH, Oh SY et al. Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer Advanced Science 1/4/2021 [PMID: 33643790]
• LE Bruijn, BEWM van den Ak, CM van Rhijn, JF Hamming, JHN Lindeman Extreme Diversity of the Human Vascular Mesenchymal Cell Landscape J Am Heart Assoc, 2020-11-16;9(23):e017094. 2020-11-16 [PMID: 33190596] (IHC, Human)
• Hiroki Arase, Noriko Sugasawa, Youhei Kawatani, Mikio Sugano, Hirotsugu Kurobe, Eiki Fujimoto et al. Appropriate Surgical Treatment of Symptomatic Primary Varicose Veins Decreases Systemic Inflammatory Biomarkers Annals of Vascular Diseases 2019-09-25 [PMID: 31636748]
• M Chen, RR Reed, AP Lane Chronic Inflammation Directs an Olfactory Stem Cell Functional Switch from Neuroregeneration to Immune Defense Cell Stem Cell, 2019-09-12;25(4):501-513.e5. 2019-09-12 [PMID: 31523027] (IHC-Fr, Mouse)
• Jenkins B D, Martini R N et al. Atypical Chemokine Receptor 1 (DARC/ACKR1) in Breast Tumors Is Associated with Survival, Circulating Chemokines, Tumor-Infiltrating Immune Cells, and African Ancestry. Cancer Epidemiol Biomarkers Prev 1/4/2019 [PMID: 30944146]
• Triulzi T, Forte L, Regondi V et al. HER2 signaling regulates the tumor immune microenvironment and trastuzumab efficacy OncoImmunology 9/21/2018 [PMID: 30546951]
• R Han, S Gu, Y Zhang, A Luo, X Jing, L Zhao, X Zhao, L Zhang Estrogen promotes progression of hormone-dependent breast cancer through CCL2-CCR2 axis by upregulation of Twist via PI3K/AKT/NF-?B signaling Sci Rep, 2018-06-22;8(1):9575. 2018-06-22 [PMID: 29934505] (IHC, Human)
• Xuefeng Deng, Wei Zou, Min Xiong, Zekun Wang, John F. Engelhardt, Shui Qing Ye et al. Human Parvovirus Infection of Human Airway Epithelia Induces Pyroptotic Cell Death by Inhibiting Apoptosis Journal of Virology 2017-12-15 [PMID: 29021400]
• Rump L, Mattey D, Kehoe O, Middleton J An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium. Cytokine, 2017-09-01;97(0):133-140. 2017-09-01 [PMID: 28648867] (IHC-Fr, Human)
• Maike Hartlage-Rübsamen, Alexander Waniek, Juliane Meissner, Markus Morawski, Stephan Schilling, Carsten Jäger et al. Isoglutaminyl cyclase contributes to CCL2-driven neuroinflammation in Alzheimer’s disease Acta Neuropathologica 2015-04-01 [PMID: 25666182]
• Bonapace L, Coissieux M, Wyckoff J, Mertz K, Varga Z, Junt T, Bentires-Alj M Cessation of CCL2 inhibition accelerates breast cancer metastasis by promoting angiogenesis. Nature, 2014-10-22;515(7525):130-3. 2014-10-22 [PMID: 25337873] (IHC-P, Human)
• Marloes Prins, Ranjan Dutta, Bart Baselmans, John J P Brevé, John G J M Bol, Sadie A Deckard et al. Discrepancy in CCL2 and CCR2 expression in white versus grey matter hippocampal lesions of Multiple Sclerosis patients Acta Neuropathologica Communications 2014-12-01 [PMID: 25149422]
• Shervin Assassi, Minghua Wu, Filemon K. Tan, Jeffrey Chang, Tiffany A. Graham, Daniel E. Furst et al. Skin Gene Expression Correlates of Severity of Interstitial Lung Disease in Systemic Sclerosis Arthritis & Rheumatism 2013-12-30 [PMID: 23897225]
• Kexiong Zhang, David J. Waxman Impact of Tumor Vascularity on Responsiveness to Anti-angiogenesis in a Prostate Cancer Stem Cell-derived Tumor Model Molecular Cancer Therapeutics 2013-05-03 [PMID: 23635653]
• Kotaro Kitaya, Tadahiro Yasuo Aberrant expression of selectin E, CXCL1, and CXCL13 in chronic endometritis Modern Pathology 2010-08-01 [PMID: 20495539]
• Colm Morrissey, Janice S. Lai, Lisha G. Brown, Ya-Chun Wang, Martine P. Roudier, Ilsa M. Coleman et al. The expression of osteoclastogenesis-associated factors and osteoblast response to osteolytic prostate cancer cells The Prostate 2010-03-01 [PMID: 19866469]
• Weigt SS, Elashoff RM, Keane MP, Strieter RM, Gomperts BN, Xue YY, Ardehali A, Gregson AL, Kubak B, Fishbein MC, Saggar R, Ross DJ, Lynch JP, Zisman DA, Belperio JA Altered levels of CC chemokines during pulmonary CMV predict BOS and mortality post-lung transplantation. Am. J. Transplant., 2008-07-01;8(7):1512-22. 2008-07-01 [PMID: 18513272] (IHC-P, Human)
• Schell C, Albrecht M, Mayer C, Schwarzer JU, Frungieri MB, Mayerhofer A Exploring human testicular peritubular cells: identification of secretory products and regulation by tumor necrosis factor-alpha. Endocrinology, 2008-01-10;149(4):1678-86. 2008-01-10 [PMID: 18187550] (ICC, Human)
• Burt D, Salvidio G, Tarabra E, Barutta F, Pinach S, Dentelli P, Camussi G, Perin PC, Gruden G The monocyte chemoattractant protein-1/cognate CC chemokine receptor 2 system affects cell motility in cultured human podocytes. Am. J. Pathol., 2007-11-30;171(6):1789-99. 2007-11-30 [PMID: 18055544] (ICC, Human)
• Pulliam L, Sun B, Rempel H Invasive chronic inflammatory monocyte phenotype in subjects with high HIV-1 viral load. J. Neuroimmunol., 2004-12-01;157(1):93-8. 2004-12-01 [PMID: 15579285] (ICC, Human)

Bioinformatics

• Gene Symbol: CCL2
• Uniprot:
  • P13500()