CCL18/PARC Antibody (64507) [Unconjugated]

• Reactivity: Hu
• Applications: WB, CyTOF-ready, ELISA(Cap), Flow
• Clone: 64507
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CCL18/PARC Antibody (64507) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CCL18/PARC
  Ala21-Ala89
  Accession # P55774.1
• Specificity: Detects human CCL18/PARC in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant human CCL3, 4, 14, 15, 23, recombinant mouse (rm) CCL3, rmCCL4, recombinant viral (rv) MIP-I or rvMIP‑II.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCL18
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Human CCL18/PARC Biotinylated Antibody (Catalog number BAF394)
  Standard: Recombinant Human CCL18/PARC (Catalog number 394-PA)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL18/PARC Antibody (64507) [Unconjugated]

• Alternative macrophage activation-associated CC chemokine 1
• AMAC-1
• AMAC1CC chemokine ligand 18
• AMAC-1Small-inducible cytokine A18
• CCL18
• chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated)
• CKb7
• DC-CK1
• DC-CK1CC chemokine PARC
• DCCK1Macrophage inflammatory protein 4
• Dendritic cell chemokine 1
• MIP4
• MIP-4
• MIP-4C-C motif chemokine 18
• PARC
• PARCPulmonary and activation-regulated chemokine
• SCYA18chemokine (C-C), dendritic
• small inducible cytokine A18
• small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary andactivation-regulated

Background

CCL18, also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein 4 (MIP-4), and dendritic cell chemokine (DC‑CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid (aa) sequence identity). CCL18 cDNA encodes an 89 aa residue precursor protein with a 20 aa putative signal peptide that is cleaved to generate a 69 aa residue mature protein which lacks potential glycosylation sites. In vitro, CCL18 mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10, and IL-13, and inhibited by IFN-gamma . CCL18 mRNA is also expressed by GM-CSF/IL-4-induced monocyte-derived dendritic cells. In vivo, CCL18 is highly expressed in lung and placenta but is not expressed in epidermal Langerhans cells. Recombinant CCL18 has been shown to chemoattract naive T cells but not monocytes or neutrophils.

1. Adema, G. et al. (1997) Nature 387:713.
2. Kodelja, V. et al. (1998) J. Immunol. 160:141.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL18/PARC Antibody (MAB394)(9)

Publications using MAB394

• SEVERIN SCHMID, AGNES CSANADI, NIKOLA KOZHUHAROV, MARCEAU TCHUDJIN, CLAUDIA KAYSER, JUSTYNA RAWLUK et al. CC-Chemokine Ligand 18 Is an Independent Prognostic Marker in Lymph Node-positive Non-small Cell Lung Cancer Anticancer Research 2018-07-01 [PMID: 29970512]
• D Huang, S Song, ZZ Wu, W Wu, X Cui, JN Chen, MS Zeng, S Su Epstein-Barr virus-induced VEGF and GM-CSF drive nasopharyngeal carcinoma metastasis via recruitment and activation of macrophages Cancer Res., 2017-05-08;0(0):. 2017-05-08 [PMID: 28484077] (IHC, Human)
• E Brencicova, AL Jagger, HG Evans, M Georgouli, A Laios, S Attard Mon, G Mehra, J Spencer, AA Ahmed, S Raju-Kanki, LS Taams, SS Diebold Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma PLoS ONE, 2017-04-14;12(4):e0175712. 2017-04-14 [PMID: 28410380] (ELISA Development (Capture), Human)
• S Su, J Liao, J Liu, D Huang, C He, F Chen, L Yang, W Wu, J Chen, L Lin, Y Zeng, N Ouyang, X Cui, H Yao, F Su, JD Huang, J Lieberman, Q Liu, E Song Blocking the recruitment of naive CD4(+) T cells reverses immunosuppression in breast cancer Cell Res, 2017-03-14;0(0):. 2017-03-14 [PMID: 28290464] (Functional Assay, ICC, IHC, Human)
• N Quillinan, KE Clark, B Youl, J Vernes, D McIntosh, S Haq, CP Denton Multiplex serum protein analysis reveals potential mechanisms and markers of response to hyperimmune caprine serum in systemic sclerosis Arthritis Res. Ther, 2017-03-07;19(1):45. 2017-03-07 [PMID: 28270187] (ELISA Development (Capture), Human)
• Miyake M, Ross S, Lawton A et al. Investigation of CCL18 and A1AT as potential urinary biomarkers for bladder cancer detection. BMC Urol. 9/5/2013 [PMID: 24011266]
• Deegan PB, Pavlova E, Tindall J Osseous manifestations of adult Gaucher disease in the era of enzyme replacement therapy. Medicine (Baltimore), 2011-01-01;90(1):52-60. 2011-01-01 [PMID: 21200186] (Luminex Development, Human)
• Pavlova EV, Deegan PB, Tindall J, McFarlane I, Mehta A, Hughes D, Wraith JE, Cox TM Potential biomarkers of osteonecrosis in Gaucher disease. Blood Cells Mol. Dis., 2010-11-13;46(1):27-33. 2010-11-13 [PMID: 21075651] (Luminex Development, Human)
• Bruna-Romero O, Schmieg J, Del Val M, Buschle M, Tsuji M The dendritic cell-specific chemokine, dendritic cell-derived CC chemokine 1, enhances protective cell-mediated immunity to murine malaria. J. Immunol., 2003-03-15;170(6):3195-203. 2003-03-15 [PMID: 12626578] (ICC, Mouse)

Bioinformatics

• Gene Symbol: CCL18
• Uniprot:
  • P55774.1()