CCL12/MCP-5 Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CCL12/MCP-5 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant mouse CCL12/MCP-5 (R&D Systems, Catalog # 428-P5)
  Gly23-Gly104
  Accession # Q62401
• Specificity: Detects mouse CCL12/MCP-5 in ELISAs and Western blots. In sandwich immunoassays, less than 0.07% cross-reactivity with recombinant human (rh) MCP‑1, rhMCP-2, rhMCP-3, rhMCP-4, recombinant mouse (rm) MIP‑1 gamma , rmJE, and rmMARC is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Ccl12
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse CCL12/MCP-5 Antibody (Catalog # MAB428)
  ELISA Detection: Mouse CCL12/MCP-5 Biotinylated Antibody (Catalog # BAF428)
  Standard:Recombinant Mouse CCL12/MCP-5 (Catalog # 428-P5)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL12/MCP-5 Antibody [Biotin]

• C-C motif chemokine 12
• CCL12
• chemokine (C-C motif) ligand 12
• MCP5
• MCP-5
• Scya12

Background

CCL12 is a cloned mouse CC chemokine most closely related to human MCP-1 (66% amino acid sequence identity in the mature protein). Mouse CCL12 encodes a 104 amino acid residue precursor protein with a 22 aa residue predicted hydrophobic signal sequence that is cleaved to generate a 82 aa residue mature protein. CCL12 is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, CCL12 expression is also induced in activated macrophages and mast cells. The gene for mouse MCP-1 has been mapped to the CC chemokine cluster on chromosome 11. Recombinant CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, CCL12 will also chemoattract eosinophils. CCL12 has been found to be a functional ligand for CCR2 but not CCR1, CCR3 or CCR5.

1. Jia, G.Q. et al. (1996) J. Exp. Med. 184:1939.
2. Sarafi, M.N. et al. (1997) J. Exp. Med. 185:99.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL12/MCP-5 Antibody (BAF428)(1)

Publication using BAF428

• Pramod Kumar Mishra, Judy M Teale Transcriptome analysis of the ependymal barrier during murine neurocysticercosis Journal of Neuroinflammation 2012-12-01 [PMID: 22731103]

Bioinformatics

• Gene Symbol: Ccl12
• Uniprot:
  • Q62401()