CCL12/MCP-5 Antibody

Recombinant Mouse CCL12/MCP‑5 (Catalog # 428-P5) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL12/MCP‑5 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL12/MCP‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF428). The ND50 is typically 0.8-4.0 µg/mL.

• Reactivity: Mu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CCL12/MCP-5 Antibody Summary

• Immunogen: E. coli-derived recombinant mouse CCL12/MCP-5 (R&D Systems, Catalog # 428-P5)
  Gly23-Gly104
  Accession # Q62401
• Specificity: Detects mouse CCL12/MCP-5 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) MCP‑3 is observed and 5% cross-reactivity with recombinant mouse (rm) JE, rmMARC, rhMCP‑1, and rhMCP‑2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Ccl12
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.8-4.0 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL12/MCP-5 Antibody

• C-C motif chemokine 12
• CCL12
• chemokine (C-C motif) ligand 12
• MCP5
• MCP-5
• Scya12

Background

CCL12 is a cloned mouse CC chemokine most closely related to human MCP-1 (66% amino acid (aa) sequence identity in the mature protein). Mouse CCL12 encodes a 104 aa residue precursor protein with a 22 aa residue predicted hydrophobic signal sequence that is cleaved to generate a 82 aa residue mature protein. CCL12 is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, CCL12 expression is also induced in activated macrophages and mast cells. At high concentrations, CCL12 will also chemoattract eosinophils. CCL12 has been found to be a functional ligand for CCR2 but not CCR1, CCR3, or CCR5.

1. Jia, G.Q. et al. (1996) J. Exp. Med. 184:1939.
2. Sarafi, M.N. et al. (1997) J. Exp. Med. 185:99.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL12/MCP-5 Antibody (AF428)(4)

Publications using AF428

• A Schlecht, J Wolf, S Boneva, G Prinz, BM Braunger, P Wieghofer, H Agostini, G Schlunck, C Lange Transcriptional and Distributional Profiling of Microglia in Retinal Angiomatous Proliferation International Journal of Molecular Sciences, 2022-03-22;23(7):. 2022-03-22 [PMID: 35408803] (Mouse)
• Lindsay A Hohsfield, Allison R Najafi, Yasamine Ghorbanian, Neelakshi Soni, Joshua Crapser, Dario X Figueroa Velez et al. Subventricular zone/white matter microglia reconstitute the empty adult microglial niche in a dynamic wave eLife 8/23/2021 [PMID: 34423781]
• Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D Comprehensive assessment of chemokine expression profiles by flow cytometry. J. Clin. Invest., 2010-02-08;120(3):907-23. 2010-02-08 [PMID: 20197626] (Flow Cytometry, Mouse)
• Szymczak WA, Deepe GS The CCL7-CCL2-CCR2 axis regulates IL-4 production in lungs and fungal immunity. J. Immunol., 2009-07-08;183(3):1964-74. 2009-07-08 [PMID: 19587014] (Neutralization, Mouse)

Review for CCL12/MCP-5 Antibody (AF428) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer B/N Mouse 11/29/2016

Summary

• Application: Block/Neutralize
• Sample Tested: fibroblasts
• Species: Mouse

Comments

• Comments: Naïve cardiac fibroblasts were stimulated with conditioned media with or without Ccl12 blocking antibody at 10 μg/mL for 24 h

Bioinformatics

• Gene Symbol: Ccl12
• Uniprot:
  • Q62401()