CCL12/MCP-5 Research Products: Novus Biologicals

Antibodies

CCL12/MCP-5 Antibody

AF428

428-P5) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL12/MCP‑5 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL12/MCP‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF428). The ND₅₀ is typically 0.8-4.0 µg/mL." class="big_lightbox" target="_blank"> Neutralization CCL12/MCP-5 Antibody

428-P5) was serially diluted and captured by Rat Anti-Mouse CCL12/MCP‑5 Monoclonal Antibody (Catalog # MAB428) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL12/MCP‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF428) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank"> ELISA CCL12/MCP-5 Antibody

(1 Review)

(4 Publications)

Species: Mu
Applications: WB, B/N
Host: Goat Polyclonal

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF428U-100UG
Alexa Fluor 405	AF428V-100UG
Alexa Fluor 488	AF428G-100UG
Alexa Fluor 532	AF428X-100UG
Alexa Fluor 594	AF428T-100UG
Alexa Fluor 647	AF428R-100UG
Alexa Fluor 700	AF428N-100UG
Alexa Fluor 750	AF428S-100UG
Alexa Fluor Plus 405	AF428AFP405-100UG
Alexa Fluor Plus 488	AF428AFP488-100UG
Alexa Fluor Plus 555	AF428AFP555-100UG
Alexa Fluor Plus 594	AF428AFP594-100UG
Alexa Fluor Plus 647	AF428AFP647-100UG
Alexa Fluor Plus 680	AF428AFP680-100UG
Allophycocyanin	AF428A
Biotin	BAF428
CoraFluor 1	AF428CL1
DyLight 350	AF428D
DyLight 405	AF428E
DyLight 488	AF428K
DyLight 550	AF428L
DyLight 594	AF428M
DyLight 650	AF428W
DyLight 680	AF428Y
DyLight 755	AF428Z
FITC	AF428F
HRP	AF428H
Janelia Fluor 525	AF428JF525
Janelia Fluor 549	AF428I
Janelia Fluor 646	AF428J
Janelia Fluor 669	AF428JF669
PE	AF428P
PerCP	AF428C
mFluor Violet 450 SE	AF428MFV450
mFluor Violet 500 SE	AF428MFV500
mFluor Violet 610 SE	AF428MFV610

Formulation	Catalog #	Availability	Price
Carrier Free	AF428-SP

CCL12/MCP-5 Antibody (63205) ...

MAB428

428-P5) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL12/ MCP-5 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL12/MCP-5 Monoclonal Antibody (Catalog # MAB428). The ND₅₀ is typically 0.5-2.5 µg/mL. Maximum neutralization observed was around 80%." class="big_lightbox" target="_blank"> Neutralization CCL12/MCP-5 Antibody (63205) [Unconjugated]

Neutralization CCL12/MCP-5 Antibody (63205) [Unconjugated]

ELISA CCL12/MCP-5 Antibody (63205) [Unconjugated]

(1 Publication)

Species: Mu
Applications: WB, B/N, ELISA(Cap)
Host: Rat Monoclonal

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB428U-100UG
Alexa Fluor 405	FAB428V-100UG
Alexa Fluor 488	FAB428G-100UG
Alexa Fluor 532	FAB428X-100UG
Alexa Fluor 594	FAB428T-100UG
Alexa Fluor 647	FAB428R-100UG
Alexa Fluor 700	FAB428N-100UG
Alexa Fluor 750	FAB428S-100UG
Alexa Fluor Plus 405	FAB428AFP405-100UG
Alexa Fluor Plus 488	FAB428AFP488-100UG
Alexa Fluor Plus 555	FAB428AFP555-100UG
Alexa Fluor Plus 594	FAB428AFP594-100UG
Alexa Fluor Plus 647	FAB428AFP647-100UG
Alexa Fluor Plus 680	FAB428AFP680-100UG
Allophycocyanin	FAB428A
Biotin	FAB428B
CoraFluor 1	FAB428CL1
DyLight 350	FAB428D
DyLight 405	FAB428E
DyLight 488	FAB428K
DyLight 550	FAB428L
DyLight 594	FAB428M
DyLight 650	FAB428W
DyLight 680	FAB428Y
DyLight 755	FAB428Z
FITC	FAB428F
HRP	FAB428H
Janelia Fluor 525	FAB428JF525
Janelia Fluor 549	FAB428I
Janelia Fluor 646	FAB428J
Janelia Fluor 669	FAB428JF669
PE	FAB428P
PerCP	FAB428C
mFluor Violet 450 SE	FAB428MFV450
mFluor Violet 500 SE	FAB428MFV500
mFluor Violet 610 SE	FAB428MFV610

Formulation	Catalog #	Availability	Price
Carrier Free	MAB428-SP
Carrier Free	MAB428-500
Carrier Free	MAB428-100

CCL12/MCP-5 Antibody (147801) ...

MAB4281

428-P5) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL12/MCP-5 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL12/MCP-5 Monoclonal Antibody (Catalog # MAB4281). The ND₅₀ is typically 0.5-2.5 µg/mL." class="big_lightbox" target="_blank"> Neutralization CCL12/MCP-5 Antibody (147801) [Unconjugated]

Neutralization CCL12/MCP-5 Antibody (147801) [Unconjugated]

Species: Mu
Applications: B/N
Host: Rat Monoclonal

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB4281U-100UG
Alexa Fluor 405	FAB4281V-100UG
Alexa Fluor 488	FAB4281G-100UG
Alexa Fluor 532	FAB4281X-100UG
Alexa Fluor 594	FAB4281T-100UG
Alexa Fluor 647	FAB4281R-100UG
Alexa Fluor 700	FAB4281N-100UG
Alexa Fluor 750	FAB4281S-100UG
Alexa Fluor Plus 405	FAB4281AFP405-100UG
Alexa Fluor Plus 488	FAB4281AFP488-100UG
Alexa Fluor Plus 555	FAB4281AFP555-100UG
Alexa Fluor Plus 594	FAB4281AFP594-100UG
Alexa Fluor Plus 647	FAB4281AFP647-100UG
Alexa Fluor Plus 680	FAB4281AFP680-100UG
Allophycocyanin	FAB4281A
Biotin	FAB4281B
CoraFluor 1	FAB4281CL1
DyLight 350	FAB4281D
DyLight 405	FAB4281E
DyLight 488	FAB4281K
DyLight 550	FAB4281L
DyLight 594	FAB4281M
DyLight 650	FAB4281W
DyLight 680	FAB4281Y
DyLight 755	FAB4281Z
FITC	FAB4281F
HRP	FAB4281H
Janelia Fluor 525	FAB4281JF525
Janelia Fluor 549	FAB4281I
Janelia Fluor 646	FAB4281J
Janelia Fluor 669	FAB4281JF669
PE	FAB4281P
PerCP	FAB4281C
mFluor Violet 450 SE	FAB4281MFV450
mFluor Violet 500 SE	FAB4281MFV500
mFluor Violet 610 SE	FAB4281MFV610