CCDC134 Antibody

Images

 
Western Blot: CCDC134 Antibody [NBP1-77053] - rat brain tissue lysate with CCDC134 antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: CCDC134 Antibody [NBP1-77053] - Immunofluorescence of CDCC134 in human brain tissue with CDCC134 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: CCDC134 Antibody [NBP1-77053] - Human brain tissue with CCDC134 antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CCDC134 Antibody Summary

Immunogen
Antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human CCDC134. The immunogen is located within the first 50 amino acids of CCDC134.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CCDC134
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Control Peptide
CCDC134 Peptide (NBP1-77053PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CCDC134 Antibody

  • CCDC134
  • coiled-coil domain containing 134
  • coiled-coil domain-containing protein 134
  • dJ821D11.3
  • FLJ22349
  • MGC21013

Background

The coiled-coil domain is a common protein motif that is often involved in protein oligomerization and is found in proteins such as transcription factors and intermediate filaments. One such protein is CCDC134, a recently identified secretory protein that has been found to inhibit the transcriptional activity of the Elk1 protein. Overexpression CCDC134 also inhibited the phosphorylation of Erk and JNK/SAPK but not p38 MAPK, while specific siRNA against CCDC134 activated Elk1 transcriptional activity and the phosphorylation of Erk and JNK/SAPK, suggesting a potential inhibiting role of CCDC134 in MAPK-mediated Elk1 transcription. CCDC134 is widely expressing in normal adult tissues, tumors, and cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CCDC134
Entrez
Uniprot