CCDC134 Antibody - BSA Free

Western Blot: CCDC134 Antibody [NBP1-77053] - rat brain tissue lysate with CCDC134 antibody at (A) 1 and (B) 2 ug/ml.

Immunocytochemistry/ Immunofluorescence: CCDC134 Antibody - BSA Free [NBP1-77053] - Immunofluorescence of CDCC134 in human brain tissue with CDCC134 antibody at 20 u/mL.

Immunohistochemistry: CCDC134 Antibody - BSA Free [NBP1-77053] - Immunohistochemistry of CCDC134 in human brain tissue with CCDC134 antibody at 2.5 u/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CCDC134 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CCDC134 Antibody - BSA Free (NBP1-77053) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human CCDC134. The immunogen is located within the first 50 amino acids of CCDC134. Amino Acid Squence: PLEIYKKMFEVKRREQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CCDC134
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1-2 ug/ml
• Control Peptide:

  CCDC134 Peptide (NBP1-77053PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for CCDC134 Antibody - BSA Free

• CCDC134
• coiled-coil domain containing 134
• coiled-coil domain-containing protein 134
• dJ821D11.3
• FLJ22349
• MGC21013

Background

The coiled-coil domain is a common protein motif that is often involved in protein oligomerization and is found in proteins such as transcription factors and intermediate filaments. One such protein is CCDC134, a recently identified secretory protein that has been found to inhibit the transcriptional activity of the Elk1 protein. Overexpression CCDC134 also inhibited the phosphorylation of Erk and JNK/SAPK but not p38 MAPK, while specific siRNA against CCDC134 activated Elk1 transcriptional activity and the phosphorylation of Erk and JNK/SAPK, suggesting a potential inhibiting role of CCDC134 in MAPK-mediated Elk1 transcription. CCDC134 is widely expressing in normal adult tissues, tumors, and cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCDC134
• Uniprot:
  • NP_079097(Human)