Ccd1/DIXDC1 Antibody

Western Blot: Ccd1/DIXDC1 Antibody [NBP1-46147] - NBP1-46147 (0.5ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Ccd1/DIXDC1 Antibody Summary

• Immunogen: Peptide with sequence C-DVSYHQVDLERE corresponding to internal region according to NP_219493.1.
• Epitope: internal region
• Specificity: This antibody is expected to recognize both isoforms (NP_001033043.1; NP_219493.1).
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DIXDC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.5 - 2 ug/ml
• Application Notes: WB: Approx. 60 kDa band observed in human heart and skeletal muscle lysates (calculated MW of 53.9 kDa band according to NP_219493.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Ccd1/DIXDC1 Antibody

• Ccd1
• Coiled-coil protein DIX1
• Coiled-coil-DIX1
• DIX domain containing 1
• DIX domain-containing protein 1
• Dixdc1
• Dixin
• Kiaa1735
• KIAA1735coiled-coil-DIX1

Background

DIXDC1 (DIX domain containing 1/Dixin) is a 683 amino acid protein. It positively regulates the Wnt signaling pathway by activating WNT3A via DVL2. It is expressed ubiquitously but has higher expression in skeletal and cardiac muscle. WNT3A signaling increases DIXDC1 protein levels by inhibiting its ubiquitination and subsequent degradation. DIXDC1 may play a role in breast cancer, breast carcinoma, endometrial cancer, epithelial neoplasia, schizophrenia, colon cancer, and neuroblastoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ccd1/DIXDC1 Antibody (NBP1-46147)(1)

Publication using NBP1-46147

• Wu Y, Jing X, Ma X et al. DIXDC1 co-localizes and interacts with gamma-tubulin in HEK293 cells. Cell Biol Int 2009-06-01 [PMID: 19375513]

Bioinformatics

• Gene Symbol: DIXDC1
• Entrez:
  • 85458(Human)
  • 330938(Mouse)
  • 363062(Rat)
• Uniprot:
  • NP_001033043.1(Human)