Ccd1/DIXDC1 Antibody [Unconjugated]

Western blot shows lysates of mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Ccd1/DIXDC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5599) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Ccd1/DIXDC1 at approximately 59 and 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Ccd1/DIXDC1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Ccd1/DIXDC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5599) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neopallial cortex and midbrain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Ccd1/DIXDC1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse Ccd1/DIXDC1
  Gln481-Asn711
  Accession # Q80Y83
• Specificity: Detects mouse Ccd1/DIXDC1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DIXDC1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ccd1/DIXDC1 Antibody [Unconjugated]

• Ccd1
• Coiled-coil protein DIX1
• Coiled-coil-DIX1
• DIX domain containing 1
• DIX domain-containing protein 1
• Dixdc1
• Dixin
• Kiaa1735
• KIAA1735coiled-coil-DIX1

Background

Coiled-coil-DIX1 (Ccd1), also known as DIXDC1, is an 80 kDa cytoplasmic actin-binding protein that mediates Wnt signaling by interacting with Dishevelled and Axin. It is highly expressed during nervous system development. Mouse Ccd1 contains a CH domain (aa 22‑152), a coiled-coil domain (aa 279‑452), and a DIX domain (aa 629‑705). Multiple alternately spliced isoforms of mouse Ccd11 are expressed in a tissue specific manner during development and in the adult. These variants may lack approximately one third of the N-terminal region of the molecule or carry short deletions within the CH domain or spacer region between the coiled-coil and DIX domains. Over aa 481‑711, mouse Ccd1 shares 88% and 75% aa sequence identity with human and rat Ccd1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ccd1/DIXDC1 Antibody (AF5599)(2)

Publications using AF5599

• Jonathan M. Goodwin, Robert U. Svensson, Hua Jane Lou, Monte M. Winslow, Benjamin E. Turk, Reuben J. Shaw A novel AMPK-independent signaling pathway downstream of the LKB1 tumor suppressor controls Snail1 and metastatic potential Molecular Cell 2014-08-07 [PMID: 25042806]
• Jonathan M. Goodwin, Robert U. Svensson, Hua Jane Lou, Monte M. Winslow, Benjamin E. Turk, Reuben J. Shaw A novel AMPK-independent signaling pathway downstream of the LKB1 tumor suppressor controls Snail1 and metastatic potential Molecular Cell 2014-08-07 [PMID: 25042806] (Western Blot, Human)

Bioinformatics

• Gene Symbol: DIXDC1
• Uniprot:
  • Q80Y83(Mouse)